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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 84014-5-RR (same clone as 84014-5-PBS)

    WB analysis using 84014-5-RR (same clone as 84014-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84014-5-RR (SRP54 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84014-5-RR (same clone as 84014-5-PBS)

    IF Staining of HeLa using 84014-5-RR (same clone as 84014-5-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SRP54 antibody (84014-5-RR, Clone: 241125D6 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84014-5-RR (same clone as 84014-5-PBS)

    FC experiment of HepG2 using 84014-5-RR (same clone as 84014-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Srp54 Recombinant Antibody (84014-5-RR, Clone:241125D6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84014-5-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00955-4

    Sandwich ELISA standard curve of MP00955-4

    Sandwich ELISA standard curve of MP00955-4, Human SRP54 Recombinant Matched Antibody Pair - PBS only. 84014-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag2327. 84014-5-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP00955-4

    Sample test of MP00955-4

    The mean SRP54 concentration was determined to be 124.39 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load.

  • Affinity and Kinetic Characterization of 84014-5-RR

    Affinity and Kinetic Characterization of 84014-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84014-5-RR against Human SRP54 were performed. The affinity constant is 0.287 nM.

SRP54 Recombinant antibody, PBS Only (Detector)

SRP54 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84014-5-PBS
CloneNo. 241125D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 241125D6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

84014-5-PBS targets SRP54 as part of a matched antibody pair:

MP00955-4: 84014-2-PBS capture and 84014-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human, mouse
Immunogen SRP54 fusion protein Ag2327 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name signal recognition particle 54kDa
Synonyms Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 241125D6
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC003389
Gene Symbol SRP54
Gene ID (NCBI) 6729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP61011
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).