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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84014-6-RR (same clone as 84014-6-PBS)

    WB analysis using 84014-6-RR (same clone as 84014-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84014-6-RR (SRP54 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84014-6-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 84014-6-RR (same clone as 84014-6-PBS)

    IF Staining of HepG2 using 84014-6-RR (same clone as 84014-6-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SRP54 antibody (84014-6-RR, Clone: 241125G1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84014-6-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 84014-6-RR (same clone as 84014-6-PBS)

    IHC staining of human placenta using 84014-6-RR (same clone as 84014-6-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84014-6-RR (SRP54 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84014-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84014-6-RR

    Affinity and Kinetic Characterization of 84014-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84014-6-RR against Human SRP54 were performed. The affinity constant is 4.24 nM.

SRP54 Recombinant antibody, PBS Only

SRP54 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 84014-6-PBS
CloneNo. 241125G1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 241125G1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

84014-6-PBS targets SRP54 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen SRP54 fusion protein Ag2327 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name signal recognition particle 54kDa
Synonyms Signal recognition particle subunit SRP54, SCN8, EC:3.6.5.4, 241125G1
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC003389
Gene Symbol SRP54
Gene ID (NCBI) 6729
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP61011
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The signal recognition particle (SRP) is a ribonucleoprotein complex that mediates the targeting of proteins to the endoplasmic reticulum (ER). The complex consists of a 7S (or 7SL) RNA and 6 different proteins, and signal recognition particle 54 (SRP54) is one of them. SRP54 binds to the signal sequence of presecretory protein as they emerge from the translating ribosomes, and then transfers them to translocating chain-associating membrane protein (TRAM).