验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
25417-1-AP targets SRPK2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | SRPK2 fusion protein Ag17885 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | SFRS protein kinase 2 |
| Synonyms | SFRS protein kinase 2, SFRSK2, SR protein specific kinase 2, SRPK2 |
| Calculated Molecular Weight | 688 aa, 78 kDa |
| Observed Molecular Weight | 105-110 kDa |
| GenBank Accession Number | BC035214 |
| Gene Symbol | SRPK2 |
| Gene ID (NCBI) | 6733 |
| RRID | AB_2880068 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78362 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SRPK2, also named as serine/arginine protein kinase 2 and SFRSK2, belongs to SR protein-specific kinase family which phosphorylate the serine/arginine domain containing proteins in the nuclear speckles to mediate pre-mRNA splicing (PMID: 22854045). In addition to its major role as kinase for the phosphorylation of SR proteins, SRPK2 is involved in cell cycle regulation and cell apoptosis (PMID: 19592491). SRPK2 promotes the proliferation and migration of the colon cancer cells. It has been reported to be regulated in several cancer types (PMID: 27041240). It has 2 isoforms with the calculated molecular mass of 77-79 kDa, and the apparent molecular mass of 105-115 kDa due to posttranslational modification (PMID: 12134018, 16122776).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SRPK2 antibody 25417-1-AP | Download protocol |
| IHC protocol for SRPK2 antibody 25417-1-AP | Download protocol |
| IP protocol for SRPK2 antibody 25417-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
