验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
25417-1-AP targets SRPK2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | SRPK2 fusion protein Ag17885 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SFRS protein kinase 2 |
| 别名 | SFRS protein kinase 2, SFRSK2, SR protein specific kinase 2, SRPK2 |
| 计算分子量 | 688 aa, 78 kDa |
| 观测分子量 | 105-110 kDa |
| GenBank蛋白编号 | BC035214 |
| 基因名称 | SRPK2 |
| Gene ID (NCBI) | 6733 |
| RRID | AB_2880068 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P78362 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SRPK2, also named as serine/arginine protein kinase 2 and SFRSK2, belongs to SR protein-specific kinase family which phosphorylate the serine/arginine domain containing proteins in the nuclear speckles to mediate pre-mRNA splicing (PMID: 22854045). In addition to its major role as kinase for the phosphorylation of SR proteins, SRPK2 is involved in cell cycle regulation and cell apoptosis (PMID: 19592491). SRPK2 promotes the proliferation and migration of the colon cancer cells. It has been reported to be regulated in several cancer types (PMID: 27041240). It has 2 isoforms with the calculated molecular mass of 77-79 kDa, and the apparent molecular mass of 105-115 kDa due to posttranslational modification (PMID: 12134018, 16122776).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SRPK2 antibody 25417-1-AP | Download protocol |
| IHC protocol for SRPK2 antibody 25417-1-AP | Download protocol |
| IP protocol for SRPK2 antibody 25417-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
