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SRPRB Polyclonal antibody

SRPRB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14636-1-AP

Print datasheet

Synonyms

APMCF1, Protein APMCF1, SR beta, SRPRB

验证数据展示

  • WB analysis using 14636-1-AP

    WB analysis using 14636-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14636-1-AP (SRPRB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 14636-1-AP

    IHC staining of human liver using 14636-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14636-1-AP (SRPRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 14636-1-AP

    IHC staining of human liver using 14636-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14636-1-AP (SRPRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inCOLO 320 cells, HepG2 cells, human liver tissue, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

14636-1-AP targets SRPRB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SRPRB fusion protein Ag6215 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal recognition particle receptor, B subunit
Synonyms APMCF1, Protein APMCF1, SR beta, SRPRB
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC065299
Gene Symbol SRPRB
Gene ID (NCBI) 58477
RRIDAB_2194868
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5M8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SRPRB antibody 14636-1-APDownload protocol
IHC protocol for SRPRB antibody 14636-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

Signal recognition particle receptor-β (SR-β) coordinates cotranslational N-glycosylation

Authors - Chatchai Phoomak
View Article