验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, HeLa cells, Jurkat cells, UV treated HEK-293 |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| CoIP | See 1 publications below |
产品信息
12822-1-AP targets SRRM1 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | SRRM1 fusion protein Ag3513 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serine/arginine repetitive matrix 1 |
| 别名 | 160 KD, POP101, SRM160, SRRM1 |
| 计算分子量 | 904 aa, 102 kDa |
| 观测分子量 | 120-160 kDa |
| GenBank蛋白编号 | BC036187 |
| 基因名称 | SRRM1 |
| Gene ID (NCBI) | 10250 |
| RRID | AB_2286613 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IYB3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SRRM1, also named as SRM160 and POP101, contains one PWI domain and belongs to the splicing factor SR family. As part of pre- and post-splicing multiprotein mRNP complexes, SRRM1 is involved in numerous pre-mRNA processing events. It promotes constitutive and exonic splicing enhancer (ESE)-dependent splicing activation by bridging together sequence-specific (SR family proteins, SFRS4, SFRS5 and TRA2B/SFRS10) and basal snRNP (SNRP70 and SNRPA1) factors of the spliceosome. SRRM1 stimulates mRNA 3'-end cleavage independently of the formation of an exon junction complex. It binds both pre-mRNA and spliced mRNA 20-25 nt upstream of exon-exon junctions. SRRM1 binds RNA and DNA with low sequence specificity and has similar preference for either double- or single-stranded nucleic acid substrates. SRRM1, a protein of high molecular weight(120-160kd), has a heavy glycosylation level and is normally highly phosphorylated. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SRRM1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SRRM1 antibody 12822-1-AP | Download protocol |
| IP protocol for SRRM1 antibody 12822-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Host Microbe Quantitative phosphoproteomics reveals extensive cellular reprogramming during HIV-1 entry.
| ||
Biochim Biophys Acta Mol Cell Res Cleaved PGAM5 dephosphorylates nuclear serine/arginine-rich proteins during mitophagy. | ||
Tissue Cell SRRM1 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by regulating the JAK/STAT signaling pathway |
