• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 20353-1-AP

    WB analysis using 20353-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20353-1-AP (SRRT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 20353-1-AP

    WB analysis of NIH/3T3 using 20353-1-AP

    WB result of SRRT antibody (20353-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRRT transfected NIH/3T3 cells.

  • WB analysis using 20353-1-AP

    WB analysis using 20353-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20353-1-AP (SRRT antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 20353-1-AP

    IF Staining of MCF-7 using 20353-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SRRT antibody (20353-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IP experiment of Jurkat using 20353-1-AP

    IP experiment of Jurkat using 20353-1-AP

    IP result of anti-SRRT (IP:20353-1-AP, 4ug; Detection:20353-1-AP 1:30000) with Jurkat cells lysate 1440 ug.

  • Featured Product
  • KD/KO验证

SRRT Polyclonal antibody

SRRT Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 20353-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, IP, ELISA

别名

serrate, ASR2, Arsenite-resistance protein 2, Arsenite resistance protein 2, ARS2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, NIH/3T3 cells, HepG2 cells, K-562 cells
Positive IP detected inJurkat cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy SRRT Ready-To-Use IHC Kit. SRRT primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20353-1-AP targets SRRT in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原 SRRT fusion protein Ag14196 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serrate RNA effector molecule homolog (Arabidopsis)
别名 serrate, ASR2, Arsenite-resistance protein 2, Arsenite resistance protein 2, ARS2
计算分子量 876 aa, 101 kDa
观测分子量 120 kDa
GenBank蛋白编号BC000082
基因名称 SRRT
Gene ID (NCBI) 51593
RRIDAB_3085603
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BXP5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SRRT (Serrate RNA effector molecule homolog) is also named as ARS2 (Arsenite-resistance protein 2). ARS2/SRRT is an essential eukaryotic protein that has emerged as a critical factor in the sorting of functional from non-functional RNA polymerase II (Pol II) transcripts (PMID: 34060620). SRRT protein is a key component of the nuclear cap-binding complex (CBC), which allows it to modulate the expression of various miRNAs. The SRRT protein is widely expressed throughout the eukaryotes, but not in budding yeast. SRRT has been shown to play a vital role in stem-cell renewal, proliferation, hematopoiesis, and RNA-mediated gene silencing and regulation of miRNA biogenesis (PMID: 37345203). SRRT is involved in cellular proliferation and colony formation (tumorigenicity). Similarly, high SRRT expression has been found in cholangiocarcinoma. ARS2 showed ubiquitous expression, with the highest levels in mouse testes, heart, and liver and human heart and skeletal muscle (PMID: 18086880).

实验方案

Product Specific Protocols
WB protocol for SRRT antibody 20353-1-APDownload protocol
IF protocol for SRRT antibody 20353-1-APDownload protocol
IP protocol for SRRT antibody 20353-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols