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  • Featured Product
  • KD/KO Validated

SRRT Polyclonal antibody

SRRT Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20353-1-AP

Print datasheet

Synonyms

serrate, ASR2, Arsenite-resistance protein 2, Arsenite resistance protein 2, ARS2

验证数据展示

  • WB analysis using 20353-1-AP

    WB analysis using 20353-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20353-1-AP (SRRT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 20353-1-AP

    WB analysis of NIH/3T3 using 20353-1-AP

    WB result of SRRT antibody (20353-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SRRT transfected NIH/3T3 cells.

  • WB analysis using 20353-1-AP

    WB analysis using 20353-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20353-1-AP (SRRT antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 20353-1-AP

    IF Staining of MCF-7 using 20353-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using SRRT antibody (20353-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IP experiment of Jurkat using 20353-1-AP

    IP experiment of Jurkat using 20353-1-AP

    IP result of anti-SRRT (IP:20353-1-AP, 4ug; Detection:20353-1-AP 1:30000) with Jurkat cells lysate 1440 ug.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, NIH/3T3 cells, HepG2 cells, K-562 cells
Positive IP detected inJurkat cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy SRRT Ready-To-Use IHC Kit. SRRT primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20353-1-AP targets SRRT in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SRRT fusion protein Ag14196 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serrate RNA effector molecule homolog (Arabidopsis)
Synonyms serrate, ASR2, Arsenite-resistance protein 2, Arsenite resistance protein 2, ARS2
Calculated Molecular Weight 876 aa, 101 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC000082
Gene Symbol SRRT
Gene ID (NCBI) 51593
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXP5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SRRT (Serrate RNA effector molecule homolog) is also named as ARS2 (Arsenite-resistance protein 2). ARS2/SRRT is an essential eukaryotic protein that has emerged as a critical factor in the sorting of functional from non-functional RNA polymerase II (Pol II) transcripts (PMID: 34060620). SRRT protein is a key component of the nuclear cap-binding complex (CBC), which allows it to modulate the expression of various miRNAs. The SRRT protein is widely expressed throughout the eukaryotes, but not in budding yeast. SRRT has been shown to play a vital role in stem-cell renewal, proliferation, hematopoiesis, and RNA-mediated gene silencing and regulation of miRNA biogenesis (PMID: 37345203). SRRT is involved in cellular proliferation and colony formation (tumorigenicity). Similarly, high SRRT expression has been found in cholangiocarcinoma. ARS2 showed ubiquitous expression, with the highest levels in mouse testes, heart, and liver and human heart and skeletal muscle (PMID: 18086880).

实验方案

Product Specific Protocols
WB protocol for SRRT antibody 20353-1-APDownload protocol
IF protocol for SRRT antibody 20353-1-APDownload protocol
IP protocol for SRRT antibody 20353-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols