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验证数据展示

  • WB analysis using 29849-1-AP

    WB analysis using 29849-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29849-1-AP (SRX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 29849-1-AP

    IHC staining of human colon cancer using 29849-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29849-1-AP (SRX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SRX1 Polyclonal antibody

SRX1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29849-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SRXN1, Sulfiredoxin-1, Sulfiredoxin 1, SRX, EC:1.8.98.2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, PC-12 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29849-1-AP targets SRX1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SRX1 fusion protein Ag31537 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sulfiredoxin 1 homolog (S. cerevisiae)
别名 SRXN1, Sulfiredoxin-1, Sulfiredoxin 1, SRX, EC:1.8.98.2
计算分子量14KD
观测分子量13-16 kDa
GenBank蛋白编号NM_080725
基因名称 SRXN1
Gene ID (NCBI) 140809
RRIDAB_2918350
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BYN0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SRX1 antibody 29849-1-APDownload protocol
IHC protocol for SRX1 antibody 29849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols