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SRX1 Polyclonal antibody

SRX1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29849-1-AP

Print datasheet

Synonyms

SRXN1, Sulfiredoxin-1, Sulfiredoxin 1, SRX, EC:1.8.98.2

验证数据展示

  • WB analysis using 29849-1-AP

    WB analysis using 29849-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29849-1-AP (SRX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 29849-1-AP

    IHC staining of human colon cancer using 29849-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29849-1-AP (SRX1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, PC-12 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29849-1-AP targets SRX1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SRX1 fusion protein Ag31537 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sulfiredoxin 1 homolog (S. cerevisiae)
Synonyms SRXN1, Sulfiredoxin-1, Sulfiredoxin 1, SRX, EC:1.8.98.2
Calculated Molecular Weight14KD
Observed Molecular Weight13-16 kDa
GenBank Accession NumberNM_080725
Gene Symbol SRXN1
Gene ID (NCBI) 140809
RRIDAB_2918350
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYN0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SRX1 antibody 29849-1-APDownload protocol
IHC protocol for SRX1 antibody 29849-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols