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验证数据展示

  • IHC staining of mouse testis using 17930-1-AP

    IHC staining of mouse testis using 17930-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17930-1-AP (SRY antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human testis using 17930-1-AP

    WB analysis of human testis using 17930-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 17930-1-AP (SRY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

SRY Polyclonal antibody

SRY Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17930-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

sex determining region Y, SRY, TDF, TDY, Testis determining factor

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue
Positive IHC detected inmouse testis tissue, human testis tissue, human spleen tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17930-1-AP targets SRY in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen SRY fusion protein Ag12360 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sex determining region Y
Synonyms sex determining region Y, SRY, TDF, TDY, Testis determining factor
Calculated Molecular Weight 204 aa, 24 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC074924
Gene Symbol SRY
Gene ID (NCBI) 6736
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05066
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SRY (sex-determining region Y protein) is a tran-scriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.