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SRY Monoclonal antibody, PBS Only

SRY Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E9A4

Cat no : 67349-1-PBS

Print datasheet

Synonyms

sex determining region Y, SRY, TDF, TDY, Testis determining factor

验证数据展示

  • WB analysis of LNCaP using 67349-1-Ig (same clone as 67349-1-PBS)

    WB analysis of LNCaP using 67349-1-Ig (same clone as 67349-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67349-1-PBS in a different storage buffer formulation.

  • WB analysis using 67349-1-Ig (same clone as 67349-1-PBS)

    WB analysis using 67349-1-Ig (same clone as 67349-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67349-1-Ig (SRY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. SRY negative cell line HeLa cell line was served as negative control. This data was developed using the same antibody clone with 67349-1-PBS in a different storage buffer formulation.

产品信息

67349-1-PBS targets SRY in WB, Indirect ELISA applications and shows reactivity with Human, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, rat
Immunogen SRY fusion protein Ag12610 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sex determining region Y
Synonyms sex determining region Y, SRY, TDF, TDY, Testis determining factor
Calculated Molecular Weight 204 aa, 24 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC074924
Gene Symbol SRY
Gene ID (NCBI) 6736
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ05066
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SRY (sex-determining region Y protein) is a tran-scriptional activator required for male sex determination in mammals. This protein, also referred to as testis-determining factor (TDF), is an HMG box protein that initiates the formation of testis from undifferentiated gonad. The DNA-binding activity of SRY is required for normal testis formation. This DNA-binding activity is thought to be regulated by PKA, which phosphorylates SRY in vivo. Mutations in SRY have been associated with 46,XY gonadal dysgenesis, in which the gonads fail to develop in XY phenotypic females.