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SSH2 Polyclonal antibody

SSH2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20674-1-AP

Print datasheet

Synonyms

hSSH 2L, KIAA1725, SSH 2, SSH 2L, SSH like protein 2, SSH2, SSH2L

验证数据展示

  • WB analysis using 20674-1-AP

    WB analysis using 20674-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20674-1-AP (SSH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 20674-1-AP

    IHC staining of human liver using 20674-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20674-1-AP (SSH2 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 20674-1-AP

    IHC staining of human liver using 20674-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20674-1-AP (SSH2 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, MG-63 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20674-1-AP targets SSH2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name slingshot homolog 2 (Drosophila)
Synonyms hSSH 2L, KIAA1725, SSH 2, SSH 2L, SSH like protein 2, SSH2, SSH2L
Calculated Molecular Weight 158 kDa
Observed Molecular Weight 45-51 kDa
GenBank Accession NumberNM_033389
Gene Symbol SSH2
Gene ID (NCBI) 85464
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ76I76
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SSH2 antibody 20674-1-APDownload protocol
IHC protocol for SSH2 antibody 20674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Adh Migr

The modulation of actin dynamics via atypical protein kinase-C activated Cofilin regulates metastasis of colorectal cancer cells.

Authors - S M Anisul Islam
View Article