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验证数据展示

  • WB analysis using 20674-1-AP

    WB analysis using 20674-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20674-1-AP (SSH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 20674-1-AP

    IHC staining of human liver using 20674-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20674-1-AP (SSH2 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 20674-1-AP

    IHC staining of human liver using 20674-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20674-1-AP (SSH2 Antibody) at dilution of 1:200 (under 40x lens).

SSH2 Polyclonal antibody

SSH2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20674-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

hSSH 2L, KIAA1725, SSH 2, SSH 2L, SSH like protein 2, SSH2, SSH2L

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, K-562 cells, MG-63 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

20674-1-AP targets SSH2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 slingshot homolog 2 (Drosophila)
别名 hSSH 2L, KIAA1725, SSH 2, SSH 2L, SSH like protein 2, SSH2, SSH2L
计算分子量 158 kDa
观测分子量 45-51 kDa
GenBank蛋白编号NM_033389
基因名称 SSH2
Gene ID (NCBI) 85464
RRIDAB_2935449
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ76I76
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SSH2 antibody 20674-1-APDownload protocol
IHC protocol for SSH2 antibody 20674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Adh Migr

The modulation of actin dynamics via atypical protein kinase-C activated Cofilin regulates metastasis of colorectal cancer cells.

Authors - S M Anisul Islam
View Article