验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HT-29 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy SSH3 Ready-To-Use IHC Kit. SSH3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
| CoIP | See 1 publications below |
产品信息
18324-1-AP targets SSH3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | IHC, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | SSH3 fusion protein Ag13174 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | slingshot homolog 3 (Drosophila) |
| 别名 | SSH3L, SSH like protein 3, SSH 3L, SSH 3, hSSH 3L |
| 计算分子量 | 659 aa, 73 kDa |
| 观测分子量 | 90-95 kDa |
| GenBank蛋白编号 | BC004210 |
| 基因名称 | SSH3 |
| Gene ID (NCBI) | 54961 |
| RRID | AB_2195490 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TE77 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SSH3 antibody 18324-1-AP | Download protocol |
| IHC protocol for SSH3 antibody 18324-1-AP | Download protocol |
| IF protocol for SSH3 antibody 18324-1-AP | Download protocol |
| IP protocol for SSH3 antibody 18324-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
