NA14 Polyclonal antibody

NA14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11797-1-AP

Print datasheet

Synonyms

N14, NA 14, NA14, Nuclear autoantigen of 14 kDa, SSNA1



经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11797-1-AP targets NA14 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen NA14 fusion protein Ag2367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Sjogren syndrome nuclear autoantigen 1
Synonyms N14, NA 14, NA14, Nuclear autoantigen of 14 kDa, SSNA1
Calculated Molecular Weight 119 aa, 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC000864
Gene Symbol SSNA1
Gene ID (NCBI) 8636
RRIDAB_2195497
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43805
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SSNA1, also named as NA14 and DIP13, may be an important adaptor to target spastin activity at the centrosome. SSNA1 is a 14 kDa protetin which locates the basal bodies and flagella of human sperm cells and the centrosome of cells.

实验方案

Product Specific Protocols
WB protocol for NA14 antibody 11797-1-APDownload protocol
IHC protocol for NA14 antibody 11797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Elife

SSNA1 stabilizes dynamic microtubules and detects microtubule damage.

Authors - Elizabeth J Lawrence
mouseIF

MBio

The cellular protein La functions in enhancement of virus release through lipid rafts facilitated by murine leukemia virus glycosylated Gag.

Authors - Nitta Takayuki T
mouseIF

Mol Biol Cell

Functional characterization of putative cilia genes by high-content analysis.

Authors - Lai Cary K CK
humanIF

PLoS One

Spastin-Interacting Protein NA14/SSNA1 Functions in Cytokinesis and Axon Development.

Authors - Uma Goyal
mouseWB

Biosci Trends

Identification of MIWI-associated Poly(A) RNAs by immunoprecipitation with an anti-MIWI monoclonal antibody.

Authors - Nishibu Takahiro T