验证数据展示
经过测试的应用
| Positive WB detected in | COS-7 cells, HepG2 cells, human liver tissue, mouse liver tissue, L02 cells |
| Positive IHC detected in | human ovary tumor tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy TRAPA/SSR1 Ready-To-Use IHC Kit. TRAPA/SSR1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10583-1-AP targets TRAPA/SSR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, monkey |
| 文献引用反应性 | human, rat, monkey |
| 免疫原 | TRAPA/SSR1 fusion protein Ag0878 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | signal sequence receptor, alpha |
| 别名 | SSR1, TRAP alpha, TRAP a, Translocon-associated protein subunit alpha, SSR-alpha |
| 计算分子量 | 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC007710 |
| 基因名称 | TRAPA |
| Gene ID (NCBI) | 6745 |
| RRID | AB_2195616 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P43307 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAPA/SSR1 antibody 10583-1-AP | Download protocol |
| IHC protocol for TRAPA/SSR1 antibody 10583-1-AP | Download protocol |
| IF protocol for TRAPA/SSR1 antibody 10583-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. | ||
Sci Adv The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress. | ||
Biosens Bioelectron Live imaging of RNA and RNA splicing in mammalian cells via the dcas13a-SunTag-BiFC system. | ||
Exp Cell Res Microdomains of endoplasmic reticulum within the sarcoplasmic reticulum of skeletal myofibers. |
