TRAPA/SSR1 Polyclonal antibody
TRAPA/SSR1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, monkey and More (1)
Applications
WB, IHC, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | COS-7 cells, HepG2 cells, human liver tissue, mouse liver tissue, L02 cells |
| Positive IHC detected in | human ovary tumor tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy TRAPA/SSR1 Ready-To-Use IHC Kit. TRAPA/SSR1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10583-1-AP targets TRAPA/SSR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, monkey samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, monkey |
| Cited Reactivity | human, rat, monkey |
| Immunogen | TRAPA/SSR1 fusion protein Ag0878 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | signal sequence receptor, alpha |
| Synonyms | SSR1, TRAP alpha, TRAP a, Translocon-associated protein subunit alpha, SSR-alpha |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC007710 |
| Gene Symbol | TRAPA |
| Gene ID (NCBI) | 6745 |
| RRID | AB_2195616 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43307 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
TRAPA (translocon-associated protein subunit alpha), also known as SSR1 (signal sequence receptor subunit alpha), is a 34-kDa single-spanning membrane glycoprotein of the endoplasmic reticulum (ER) (PMID: 8050590). It is a part of the tetrameric TRAP complex (TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-gamma) which functions in regulating the retention of ER resident proteins (PMID: 7916687). The TRAP complex is also involved in endoplasmic reticulum-associated degradation (ERAD) (PMID: 17380188).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAPA/SSR1 antibody 10583-1-AP | Download protocol |
| IHC protocol for TRAPA/SSR1 antibody 10583-1-AP | Download protocol |
| IF protocol for TRAPA/SSR1 antibody 10583-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. | ||
Sci Adv The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress. | ||
Biosens Bioelectron Live imaging of RNA and RNA splicing in mammalian cells via the dcas13a-SunTag-BiFC system. | ||
Exp Cell Res Microdomains of endoplasmic reticulum within the sarcoplasmic reticulum of skeletal myofibers. |
