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验证数据展示

  • WB analysis using 11655-2-AP

    WB analysis using 11655-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11655-2-AP (SSR4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11655-2-AP

    IHC staining of human pancreas cancer using 11655-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11655-2-AP (SSR4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 11655-2-AP

    IP experiment of HeLa using 11655-2-AP

    IP result of anti-SSR4 (IP:11655-2-AP, 4ug; Detection:11655-2-AP 1:300) with HeLa cells lysate 2600 ug.

  • IF Staining of HeLa using 11655-2-AP

    IF Staining of HeLa using 11655-2-AP

    Immunofluorescent analysis of HeLa cells using 11655-2-AP (SSR4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SSR4 Polyclonal antibody

SSR4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11655-2-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

TRAP-delta, TRAPD, Translocon-associated protein subunit delta, SSR-delta

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, human placenta tissue, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11655-2-AP targets SSR4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 SSR4 fusion protein Ag2250 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 signal sequence receptor, delta (translocon-associated protein delta)
别名 TRAP-delta, TRAPD, Translocon-associated protein subunit delta, SSR-delta
计算分子量 173 aa, 19 kDa
观测分子量 19 kDa
GenBank蛋白编号BC003371
基因名称 SSR4
Gene ID (NCBI) 6748
RRIDAB_2195738
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51571
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Signal sequence receptor subunit delta (SSR4, or TRAPD) gene encodes the translocon-associated proteins subunit delta, which is about 19-kDa and is located at endoplasmic reticulum (ER) membrane, the translocon-associated proteins complex functions to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. TRAP-delta can form heterotetramer with other TRAP homolog TRAP-alpha, TRAP-beta and TRAP-gamma.

实验方案

Product Specific Protocols
WB protocol for SSR4 antibody 11655-2-APDownload protocol
IHC protocol for SSR4 antibody 11655-2-APDownload protocol
IF protocol for SSR4 antibody 11655-2-APDownload protocol
IP protocol for SSR4 antibody 11655-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

The translocon-associated protein (TRAP) complex regulates quality control of N-linked glycosylation during ER stress.

Authors - Chatchai Phoomak
View Article
humanIHC

Expert Rev Mol Diagn

SSR4 as a prognostic biomarker and related with immune infiltration cells in colon adenocarcinoma.

Authors - Weiwei He
View Article
humanIHC

Cancer Med

Predicting Outcomes in Esophageal Squamous Cell Carcinoma Using scRNA-Seq and Bulk RNA-Seq: A Model Development and Validation Study

Authors - Jiaqi Zhang
View Article