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SSRP1 Polyclonal antibody

SSRP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15696-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

FACT80, FACT 80 kDa subunit, FACT, Facilitates chromatin transcription complex subunit SSRP1, Facilitates chromatin transcription complex 80 kDa subunit

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SSRP1 Ready-To-Use IHC Kit. SSRP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15696-1-AP targets SSRP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 SSRP1 fusion protein Ag8318 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 structure specific recognition protein 1
别名 FACT80, FACT 80 kDa subunit, FACT, Facilitates chromatin transcription complex subunit SSRP1, Facilitates chromatin transcription complex 80 kDa subunit
计算分子量 81 kDa
观测分子量 86 kDa
GenBank蛋白编号BC005116
基因名称 SSRP1
Gene ID (NCBI) 6749
RRIDAB_2195756
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ08945
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SSRP1 antibody 15696-1-APDownload protocol
IHC protocol for SSRP1 antibody 15696-1-APDownload protocol
IF protocol for SSRP1 antibody 15696-1-APDownload protocol
IP protocol for SSRP1 antibody 15696-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Transl Med

Therapeutic targeting of the MYC signal by inhibition of histone chaperone FACT in neuroblastoma.

Authors - Daniel R Carter
humanWB

ACS Appl Mater Interfaces

Injectable Bacteria-Sensitive Hydrogel Promotes Repair of Infected Fractures via Sustained Release of miRNA Antagonist.

Authors - Chenyan Yu
  • KD Validated
mouseIHC

FASEB J

Chronic exercise training activates histone turnover in mouse skeletal muscle fibers.

Authors - Ikumi Ohsawa
humanWB,IHC

Int J Med Sci

SSRP1 influences colorectal cancer cell growth and apoptosis via the AKT pathway.

Authors - Qian Wang
  • KD Validated
humanWB

Cell Rep

TRPS1 drives heterochromatic origin refiring and cancer genome evolution.

Authors - Jianguo Yang
humanWB,IHC

J Cell Mol Med

SSRP1 promotes colorectal cancer progression and is negatively regulated by miR-28-5p.

Authors - Wei Wu
  • KD Validated
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