验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse testis tissue |
Positive IP detected in | mouse testis tissue |
Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 3 publications below |
WB | See 5 publications below |
IHC | See 3 publications below |
产品信息
15696-1-AP targets SSRP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | SSRP1 fusion protein Ag8318 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | structure specific recognition protein 1 |
别名 | FACT80, FACT 80 kDa subunit, FACT, Facilitates chromatin transcription complex subunit SSRP1, Facilitates chromatin transcription complex 80 kDa subunit |
计算分子量 | 81 kDa |
观测分子量 | 86 kDa |
GenBank蛋白编号 | BC005116 |
基因名称 | SSRP1 |
Gene ID (NCBI) | 6749 |
RRID | AB_2195756 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q08945 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for SSRP1 antibody 15696-1-AP | Download protocol |
IHC protocol for SSRP1 antibody 15696-1-AP | Download protocol |
IF protocol for SSRP1 antibody 15696-1-AP | Download protocol |
IP protocol for SSRP1 antibody 15696-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Transl Med Therapeutic targeting of the MYC signal by inhibition of histone chaperone FACT in neuroblastoma. | ||
ACS Appl Mater Interfaces Injectable Bacteria-Sensitive Hydrogel Promotes Repair of Infected Fractures via Sustained Release of miRNA Antagonist.
| ||
FASEB J Chronic exercise training activates histone turnover in mouse skeletal muscle fibers. | ||
Int J Med Sci SSRP1 influences colorectal cancer cell growth and apoptosis via the AKT pathway.
| ||
J Cell Mol Med SSRP1 promotes colorectal cancer progression and is negatively regulated by miR-28-5p.
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