验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy SSRP1 Ready-To-Use IHC Kit. SSRP1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 5 publications below |
| IHC | See 3 publications below |
产品信息
15696-1-AP targets SSRP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | SSRP1 fusion protein Ag8318 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | structure specific recognition protein 1 |
| Synonyms | FACT80, FACT 80 kDa subunit, FACT, Facilitates chromatin transcription complex subunit SSRP1, Facilitates chromatin transcription complex 80 kDa subunit |
| Calculated Molecular Weight | 81 kDa |
| Observed Molecular Weight | 86 kDa |
| GenBank Accession Number | BC005116 |
| Gene Symbol | SSRP1 |
| Gene ID (NCBI) | 6749 |
| RRID | AB_2195756 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q08945 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SSRP1 antibody 15696-1-AP | Download protocol |
| IHC protocol for SSRP1 antibody 15696-1-AP | Download protocol |
| IF protocol for SSRP1 antibody 15696-1-AP | Download protocol |
| IP protocol for SSRP1 antibody 15696-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Transl Med Therapeutic targeting of the MYC signal by inhibition of histone chaperone FACT in neuroblastoma. | ||
ACS Appl Mater Interfaces Injectable Bacteria-Sensitive Hydrogel Promotes Repair of Infected Fractures via Sustained Release of miRNA Antagonist.
| ||
FASEB J Chronic exercise training activates histone turnover in mouse skeletal muscle fibers. | ||
Int J Med Sci SSRP1 influences colorectal cancer cell growth and apoptosis via the AKT pathway.
| ||
J Cell Mol Med SSRP1 promotes colorectal cancer progression and is negatively regulated by miR-28-5p.
|
