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验证数据展示

  • IHC staining of mouse testis using 12273-1-AP

    IHC staining of mouse testis using 12273-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12273-1-AP (SSSCA1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 12273-1-AP

    IHC staining of mouse testis using 12273-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12273-1-AP (SSSCA1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 12273-1-AP

    WB analysis of HeLa using 12273-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12273-1-AP (SSSCA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 12273-1-AP

    IP experiment of HeLa using 12273-1-AP

    IP result of anti-SSSCA1 (IP:12273-1-AP, 4ug; Detection:12273-1-AP 1:1000) with HeLa cells lysate 2000ug.

  • FC experiment of HeLa using 12273-1-AP

    FC experiment of HeLa using 12273-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SSSCA1 Polyclonal antibody (12273-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

SSSCA1 Polyclonal antibody

SSSCA1 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA
Cat No. 12273-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), IP, ELISA

别名

ZNRD2, Zinc ribbon domain-containing protein 2, Protein ZNRD2, p27, Autoantigen p27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12273-1-AP targets SSSCA1 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 SSSCA1 fusion protein Ag2916 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Sjogren syndrome/scleroderma autoantigen 1
别名 ZNRD2, Zinc ribbon domain-containing protein 2, Protein ZNRD2, p27, Autoantigen p27
计算分子量 199 aa, 21 kDa
观测分子量 27 kDa
GenBank蛋白编号BC014791
基因名称 SSSCA1
Gene ID (NCBI) 10534
RRIDAB_2195902
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60232
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SSSCA1, also named as Autoantigen p27, is centromere-associated protein. It may induce anti-centromere antibodies. SSSCA1 plays a role in mitosis. It's involved in Sjogren syndrome/scleroderma. The calculated MW of SSSCA1 is 21.5 kDa (native form), with modification the MW is about23- 27 kDa.

实验方案

Product Specific Protocols
WB protocol for SSSCA1 antibody 12273-1-APDownload protocol
IHC protocol for SSSCA1 antibody 12273-1-APDownload protocol
IP protocol for SSSCA1 antibody 12273-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

Mechanism of orphan subunit recognition during assembly quality control

Authors - Yuichi Yagita
  • KO Validated
View Article
human,mouseWB,IHC

J Ethnopharmacol

Centipeda minima extracts and the active sesquiterpene lactones have therapeutic efficacy in non-small cell lung cancer by suppressing Skp2/p27 signaling pathway

Authors - Han-Chen Wang
View Article