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验证数据展示

  • IHC staining of mouse pancreas using 84351-1-RR

    IHC staining of mouse pancreas using 84351-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84351-1-RR (SST-Specific antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 84351-1-RR

    IHC staining of mouse pancreas using 84351-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84351-1-RR (SST-Specific antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse pancreas using 84351-1-RR

    IF Staining of mouse pancreas using 84351-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Somatostatin antibody (84351-1-RR, Clone: 240816D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse pancreas using 84351-1-RR

    IF Staining of mouse pancreas using 84351-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using Somatostatin antibody (84351-1-RR, Clone: 240816D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Somatostatin Recombinant antibody

Somatostatin Recombinant Antibody for IHC, IF-P, ELISA
Cat No. 84351-1-RR

产品说明书

CloneNo. 240816D9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF-P, ELISA

别名

SST, somatostatin, SST, SMST, Growth hormone release-inhibiting factor, 240816D9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84351-1-RR targets Somatostatin in IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 somatostatin
别名 SST, somatostatin, SST, SMST, Growth hormone release-inhibiting factor, 240816D9
计算分子量 13 kDa
GenBank蛋白编号NM_001048
基因名称 Somatostatin
Gene ID (NCBI) 6750
RRIDAB_3671889
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP61278
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Somatostatin is a peptide hormone that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction with G protein-coupled somatostatin receptors and inhibition of the release of numerous secondary hormones. Somatostatin is a useful marker of D-cells of pancreatic islet cells. Somatostatin inhibits ins and glucagon secretion. Somatostatin has two active forms produced by alternative cleavage of a single preproprotein: one of 14 amino acids, the other of 28 amino acids.

实验方案

Product Specific Protocols
IHC protocol for Somatostatin antibody 84351-1-RRDownload protocol
IF protocol for Somatostatin antibody 84351-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols