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SSTR1 Polyclonal antibody

SSTR1 Polyclonal Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA, FC (Intra)

Conjugate

Unconjugated

Cat no : 20587-1-AP

Print datasheet

Synonyms

SS1 R, SS 1 R, SRIF-2, SRIF 2, Somatostatin receptor type 1

验证数据展示

  • WB analysis of L02 using 20587-1-AP

    WB analysis of L02 using 20587-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20587-1-AP (SSTR1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • FC experiment of SH-SY5Y using 20587-1-AP

    FC experiment of SH-SY5Y using 20587-1-AP

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug SSTR1 Polyclonal antibody (20587-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inL02 cells, BxPC-3 cells, mouse brain tissue, mouse small intestine tissue, SH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
Positive FC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20587-1-AP targets SSTR1 in WB, ELISA, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen SSTR1 fusion protein Ag14287 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name somatostatin receptor 1
Synonyms SS1 R, SS 1 R, SRIF-2, SRIF 2, Somatostatin receptor type 1
Calculated Molecular Weight 391 aa, 43 kDa
Observed Molecular Weight 53 kDa, 63 kDa
GenBank Accession NumberBC035618
Gene Symbol SSTR1
Gene ID (NCBI) 6751
RRIDAB_10755146
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30872
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SSTR1 antibody 20587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols