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验证数据展示

  • IHC staining of mouse stomach using 20404-1-AP

    IHC staining of mouse stomach using 20404-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 20404-1-AP (SSTR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 20404-1-AP

    IHC staining of mouse pancreas using 20404-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20404-1-AP (SSTR2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas using 20404-1-AP

    IHC staining of human pancreas using 20404-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 20404-1-AP (SSTR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Neuro-2a using 20404-1-AP

    IF Staining of Neuro-2a using 20404-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using SSTR2 antibody (20404-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SSTR2 Polyclonal antibody

SSTR2 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 20404-1-AP

Print datasheet

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, ELISA and More (1)

Synonyms

SS2 R, SS 2 R, SRIF-1, SRIF 1, Somatostatin receptor type 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inmouse pancreas tissue, human pancreas tissue, human kidney tissue, mouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNeuro-2a cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20404-1-AP targets SSTR2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IF/ICC, ELISA Application Description
Cited ApplicationsIHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen SSTR2 fusion protein Ag14205 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name somatostatin receptor 2
Synonyms SS2 R, SS 2 R, SRIF-1, SRIF 1, Somatostatin receptor type 2
Calculated Molecular Weight 369 aa, 41 kDa
GenBank Accession NumberBC019610
Gene Symbol SSTR2
Gene ID (NCBI) 6752
RRIDAB_2878683
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30874
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Somatostatin receptor 2 (SSTR2) is a G-protein coupled membrane receptor expressed in a variety of neural and neurosecretory tissue types. In neuroendocrine tumors (NETs), in which Somatostatin receptors (SSTRs) are commonly expressed, the detection of SSTR2 expression has both diagnostic and therapeutic potential. Besides NETs, SSTR2 immunostaining can be detected in other types of tumor cells(PMID:34786053). Moreover, SSTR2 is consistently expressed in Olfactory neuroblastoma (ONB) suggesting a role for somatostatin-analog based imaging and therapy in this disease. More generally, SSTR2 may be another marker of neuroendocrine diferentiation in ONB(PMID:33929681). SSTR2 has 2 isoforms with the MW of 40-41 kDa.

实验方案

Product Specific Protocols
IHC protocol for SSTR2 antibody 20404-1-APDownload protocol
IF protocol for SSTR2 antibody 20404-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Appl Radiat Isot

Autoradiography on deparaffinized tissue sections - A feasibility study with 68Ga-labeled PET-tracers

Authors - Eva-Maria Klebermass
View Article
mouseIF

Acta Neuropathol Commun

Abnormal accumulation of extracellular vesicles in hippocampal dystrophic axons and regulation by the primary cilia in Alzheimer's disease

Authors - Jaemyung Jang
View Article