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验证数据展示

  • WB analysis using 15434-1-AP

    WB analysis using 15434-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 15434-1-AP (SSU72 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 15434-1-AP

    IHC staining of human liver using 15434-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with SSU72 antibody included
  • IHC staining of human liver using 15434-1-AP

    IHC staining of human liver using 15434-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15434-1-AP (SSU72 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with SSU72 antibody included

SSU72 Polyclonal antibody

SSU72 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15434-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

RNA polymerase II subunit A C-terminal domain phosphatase SSU72, PNAS-120, PNAS 120, HSPC182, EC:3.1.3.16

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells
Positive IHC detected inhuman liver tissue, human breast cancer tissue, human prostate cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SSU72 Ready-To-Use IHC Kit. SSU72 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15434-1-AP targets SSU72 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SSU72 fusion protein Ag7699 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae)
Synonyms RNA polymerase II subunit A C-terminal domain phosphatase SSU72, PNAS-120, PNAS 120, HSPC182, EC:3.1.3.16
Calculated Molecular Weight 23 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC008070
Gene Symbol SSU72
Gene ID (NCBI) 29101
RRIDAB_2878138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP77
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SSU72 antibody 15434-1-APDownload protocol
IHC protocol for SSU72 antibody 15434-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols