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验证数据展示

  • WB analysis of MCF-7 using 15876-1-AP

    WB analysis of MCF-7 using 15876-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 15876-1-AP (SSX4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 15876-1-AP

    IHC staining of human testis using 15876-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15876-1-AP (SSX4 Antibody) at dilution of 1:200 (under 10x lens).

SSX4 Polyclonal antibody

SSX4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15876-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

Cancer/testis antigen 5.4, CT5.4, Protein SSX4, SSX4, SSX4A, SSX4B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells, A549 cells, K-562 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15876-1-AP targets SSX4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen SSX4 fusion protein Ag8637 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name synovial sarcoma, X breakpoint 4
Synonyms Cancer/testis antigen 5.4, CT5.4, Protein SSX4, SSX4, SSX4A, SSX4B
Calculated Molecular Weight 188 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC005325
Gene Symbol SSX4
Gene ID (NCBI) 6759
RRIDAB_2035018
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60224
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SSX4 antibody 15876-1-APDownload protocol
IHC protocol for SSX4 antibody 15876-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols