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验证数据展示

  • WB analysis of MCF-7 using 27176-1-AP

    WB analysis of MCF-7 using 27176-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27176-1-AP (ST14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 27176-1-AP

    IHC staining of human colon using 27176-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human colon using 27176-1-AP

    IHC staining of human colon using 27176-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 27176-1-AP (ST14 antibody) at dilution of 1:200 (under 40x lens).

ST14 Polyclonal antibody

ST14 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27176-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA and More (2)

别名

HAI, Matriptase, MT SP1, MTSP 1, MTSP1, Prostamin, PRSS14, Serine protease 14, Serine protease TADG 15, SNC19, ST14, TADG 15, TADG15

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman colon tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27176-1-AP targets ST14 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF, IHC, CoIP
经测试反应性 human
文献引用反应性human
免疫原 ST14 fusion protein Ag26007 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 suppression of tumorigenicity 14 (colon carcinoma)
别名 HAI, Matriptase, MT SP1, MTSP 1, MTSP1, Prostamin, PRSS14, Serine protease 14, Serine protease TADG 15, SNC19, ST14, TADG 15, TADG15
计算分子量 46 kDa
观测分子量 100 kDa
GenBank蛋白编号BC030532
基因名称 ST14
Gene ID (NCBI) 6768
RRIDAB_2880788
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y5Y6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Suppression of tumorigenicity 14(ST14), is an epithelial-derived, integral membrane serine protease. ST14 forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. ST14 can cleave and activate hepatocyte growth factor/scattering factor, and uPA, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors.

实验方案

Product Specific Protocols
WB protocol for ST14 antibody 27176-1-APDownload protocol
IHC protocol for ST14 antibody 27176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF,CoIP

BMC Cancer

ST14 interacts with TMEFF1 and is a predictor of poor prognosis in ovarian cancer

Authors - Xin Nie
View Article