ST3GAL2 Polyclonal antibody
ST3GAL2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human colon tissue, HL-60 cells, human heart tissue, human liver tissue, human spleen tissue, K-562 cells |
| Positive IHC detected in | rat heart tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
13620-1-AP targets ST3GAL2 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, rat |
| Cited Reactivity | human, mouse |
| Immunogen | ST3GAL2 fusion protein Ag4528 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ST3 beta-galactoside alpha-2,3-sialyltransferase 2 |
| Synonyms | Alpha 2,3 ST 2, Gal NAc6S, Sialyltransferase 4B, SIAT4 B, SIAT4B, ST3Gal II, ST3GAL2, ST3GalA.2, ST3GALII |
| Calculated Molecular Weight | 350 aa, 40 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC036777 |
| Gene Symbol | ST3GAL2 |
| Gene ID (NCBI) | 6483 |
| RRID | AB_2286561 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16842 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2) is a sialyltransferase (ST) that transfers sialic acid from the activated donor CMP-sialic acid to the glycan terminus of a glycolipid or glycoprotein. ST3GAL2 contains two putative N-glycosylation sites (Asn92 and Asn211), which are mainly responsible for GD1a and GT1b ganglioside biosynthesis in the brain. ST3GAL2 belongs to the sialyltransferase family, which mediates the terminal modification of glycoproteins and glycolipids. ST3GAL2 has been found to play a role in obesity, aging, and malignant diseases (PMID: 34319453). The calculated molecular weight of ST3GAL2 is 40 kDa, but the observed is 55 kDa, which may be the glycosylation form of ST3GAL2.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ST3GAL2 antibody 13620-1-AP | Download protocol |
| IHC protocol for ST3GAL2 antibody 13620-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
