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  • Featured Product
  • KD/KO Validated

ST3GAL3 Polyclonal antibody

ST3GAL3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24925-1-AP

Print datasheet

Synonyms

Alpha 2,3 ST 3, Sialyltransferase 6, SIAT6, ST3Gal III, ST3GAL3, ST3GALII, ST3GalIII, ST3N

验证数据展示

  • WB analysis of mouse liver using 24925-1-AP

    WB analysis of mouse liver using 24925-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24925-1-AP (ST3GAL3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 24925-1-AP

    IHC staining of human skin using 24925-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 24925-1-AP (ST3GAL3 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human skin using 24925-1-AP

    IHC staining of human skin using 24925-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 24925-1-AP (ST3GAL3 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24925-1-AP is ST3GAL3 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ST3GAL3 fusion protein Ag20795 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ST3 beta-galactoside alpha-2,3-sialyltransferase 3
Synonyms Alpha 2,3 ST 3, Sialyltransferase 6, SIAT6, ST3Gal III, ST3GAL3, ST3GALII, ST3GalIII, ST3N
Calculated Molecular Weight 375 aa, 42 kDa
Observed Molecular Weight 44-55 kDa
GenBank Accession NumberBC050380
Gene Symbol ST3GAL3
Gene ID (NCBI) 6487
RRIDAB_2879799
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ11203
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ST3GAL3 antibody 24925-1-APDownload protocol
IHC protocol for ST3GAL3 antibody 24925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

FEBS Lett

The sialyltransferase ST3Gal3 facilitates the receptivity of the uterine endometrium in vitro and in vivo.

Authors - Ming Yu
  • KD Validated
View Article