验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Jurkat cells, HeLa cells, human testis tissue, human placenta tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, human skeletal muscle tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14614-1-AP targets ST3GAL5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | ST3GAL5 fusion protein Ag6207 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ST3 beta-galactoside alpha-2,3-sialyltransferase 5 |
| 别名 | ST3GAL5, ST3GalV, UNQ2510/PRO5998, CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase, EC:2.4.3.9, Lactosylceramide alpha-2,3-sialyltransferase, SIAT9, SIATGM3S, ST3Gal V |
| 计算分子量 | 48 kDa |
| 观测分子量 | 45-50 kDa |
| GenBank蛋白编号 | BC065936 |
| 基因名称 | ST3GAL5 |
| Gene ID (NCBI) | 8869 |
| RRID | AB_2194414 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UNP4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ST3GAL5 is also named as SIAT9, GM3 synthase, ST3Gal V, Sialyltransferase 9 and belongs to the glycosyltransferase 29 family. ST3GAL5 transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc to the non-reducing terminal galactose of glycosphingolipids forming gangliosides (PMID:9822625, PMID:16934889). SIAT9 is mainly involved in the biosynthesis of ganglioside GM3 but can also use different glycolipids as substrate acceptors such as D-galactosylceramide (GalCer), asialo-GM2 (GA2) and asialo-GM1 (GA1) (PMID:16934889). ST3GAL5 is highly expressed in brain, skeletal muscle, placenta, and testis. And mRNA of ST3GAL5 is widely distributed in human brain, but slightly elevated expression was observed in the cerebral cortex, temporal lobe, and putamen (PMID:9822625). ST3GAL5 has 3 isoforms with the molecular mass of 45, 46, and 48 kDa. Sometimes the band of 70 kDa can also be observed, which may be a glycosylated form of ST3GAL5 (PMID: 34943806, PMID: 26458842).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ST3GAL5 antibody 14614-1-AP | Download protocol |
| IHC protocol for ST3GAL5 antibody 14614-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Immunol Identification of ST3GAL5 as a prognostic biomarker correlating with CD8+ T cell exhaustion in clear cell renal cell carcinoma | ||
Front Cell Dev Biol PRC2-dependent regulation of ganglioside expression during dedifferentiation contributes to the proliferation and migration of vascular smooth muscle cells | ||
iScience C-Myc-induced hypersialylation of small cell lung cancer facilitates pro-tumoral phenotypes of macrophages | ||
Mol Oncol Exosomes secreted by ST3GAL5high cancer cells promote peritoneal dissemination by establishing a pre-metastatic microenvironment
| ||
Int Immunopharmacol Identification and validation of sialyltransferase ST3Gal5 in bladder cancer through bioinformatics and experimental analysis |
