ST6GAL1 Monoclonal antibody
ST6GAL1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, pig
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1F11G5
验证数据展示
经过测试的应用
| Positive WB detected in | TF-1 cells |
Planning an IHC experiment? We recommend our IHCeasy ST6GAL1 Ready-To-Use IHC Kit. ST6GAL1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68633-1-Ig targets ST6GAL1 in WB, ELISA applications and shows reactivity with human, pig samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | human, pig |
| Immunogen | ST6GAL1 fusion protein Ag6181 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ST6 beta-galactosamide alpha-2,6-sialyltranferase 1 |
| Synonyms | CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1, CD75, Beta-galactoside alpha-2,6-sialyltransferase 1, B-cell antigen CD75, Alpha 2,6-ST 1 |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 43-45 kDa, 50-70 kDa |
| GenBank Accession Number | BC040009 |
| Gene Symbol | ST6GAL1 |
| Gene ID (NCBI) | 6480 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P15907 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ST6GAL1 (β-galactoside α-2-6 sialyl transferase1; also known as ST6N or CD75) is a sialyltransferase mediating the glycosylation of proteins and lipids to form functionally important glycoproteins and glycolipids in the Golgi compartment. It is principally expressed in liver, placenta and skeletal muscle. ST6GAL1 undergoes proteolytic process to generate soluble form from membrane form. Western blot analysis of human liver using this antibody detects both isoforms between 43-50 kDa. Higher molecular weight of bands around 50-70 kDa can also be observed with unknown reason. (PMID: 15049997)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ST6GAL1 antibody 68633-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |