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ST6GALNAC6 Monoclonal antibody, PBS Only

ST6GALNAC6 Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

1A7B7

Cat No : 66209-1-PBS

Print datasheet

Synonyms

hST6GalNAc VI, RP11 203J24.3, Sialyltransferase 7F, SIAT7 F, SIAT7F, ST6GalNAc VI, ST6GALNAC6, ST6GALNACVI

验证数据展示

  • IF Staining of human colon cancer using 66209-1-Ig (same clone as 66209-1-PBS)

    IF Staining of human colon cancer using 66209-1-Ig (same clone as 66209-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using ST6GALNAC6 antibody (66209-1-Ig, Clone: 1A7B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

  • WB analysis of COLO 320 using 66209-1-Ig (same clone as 66209-1-PBS)

    WB analysis of COLO 320 using 66209-1-Ig (same clone as 66209-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 66209-1-Ig (same clone as 66209-1-PBS)

    IHC staining of mouse colon using 66209-1-Ig (same clone as 66209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 66209-1-Ig (same clone as 66209-1-PBS)

    IHC staining of mouse colon using 66209-1-Ig (same clone as 66209-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66209-1-Ig (ST6GALNAC6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66209-1-PBS in a different storage buffer formulation.

产品信息

66209-1-PBS targets ST6GALNAC6 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB,IHC,IF,ELISA Application Description
Tested Reactivity human, mouse
Immunogen ST6GALNAC6 fusion protein Ag8358 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6
Synonyms hST6GalNAc VI, RP11 203J24.3, Sialyltransferase 7F, SIAT7 F, SIAT7F, ST6GalNAc VI, ST6GALNAC6, ST6GALNACVI
Calculated Molecular Weight333aa,38 kDa; 374aa,41 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC007802
Gene Symbol ST6GALNAC6
Gene ID (NCBI) 30815
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ969X2
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

背景介绍

ST6GALNAC6 is a sialyltransferase which is responsible for the synthesis of all a-series gangliosides. It has a broad expression pattern. ST6GALNAC6 was reported to be a key enzyme in the synthesis of disialyl monosialyl Lewis (Lea), a representative tumor-associated antigen in cancers of the pancreas and colon.