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ST7 Polyclonal antibody

ST7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11945-1-AP

Print datasheet

Synonyms

ETS7q, FAM4A, FAM4A1, HELG, Protein FAM4A1, Protein HELG, RAY1, SEN4, ST7, TSG7

验证数据展示

  • WB analysis using 11945-1-AP

    WB analysis using 11945-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11945-1-AP (ST7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 11945-1-AP

    IHC staining of human liver cancer using 11945-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11945-1-AP (ST7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, Jurkat cells, PC-3 cells
Positive IHC detected inhuman liver cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11945-1-AP targets ST7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ST7 fusion protein Ag2547 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name suppression of tumorigenicity 7
Synonyms ETS7q, FAM4A, FAM4A1, HELG, Protein FAM4A1, Protein HELG, RAY1, SEN4, ST7, TSG7
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC030954
Gene Symbol ST7
Gene ID (NCBI) 7982
RRIDAB_2270936
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRC1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human ST7 (suppressor of tumorigenicity 7) gene, also called FAM4A1, HELG or RAY1, maps to a region on chromosome 7 identified as an autism-susceptibility locus. ST7 has been proposed to be a tumor suppressor gene with highest expression in heart, liver and pancreas. The product of this gene is a multi-pass membrane protein belonging to the ST7 family, full-length ST7 cDNA has been cloned and verified in cancer cell lines, recent result suggests that ST7 may mediate tumor suppression through the regulation of the genes involved in maintaining the cellular structure of the cell and involved in oncogenic pathways.

实验方案

Product Specific Protocols
WB protocol for ST7 antibody 11945-1-APDownload protocol
IHC protocol for ST7 antibody 11945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Res

Genetic validation of the protein arginine methyltransferase PRMT5 as a candidate therapeutic target in glioblastoma.

Authors - Fengting Yan
View Article