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ST8SIA3 Polyclonal antibody

ST8SIA3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18004-1-AP

Print datasheet

Synonyms

Alpha 2,8 sialyltransferase 8C, Sialyltransferase 8C, Sialytransferase St8Sia III, SIAT8 C, SIAT8C, ST8SIA3, ST8SiaIII

验证数据展示

  • WB analysis using 18004-1-AP

    WB analysis using 18004-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18004-1-AP (ST8SIA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 18004-1-AP

    IHC staining of human liver using 18004-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 18004-1-AP (ST8SIA3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 18004-1-AP

    IHC staining of human liver using 18004-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 18004-1-AP (ST8SIA3 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inA549 cells, mouse liver tissue, Caco-2 cells
Positive IHC detected inhuman liver tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18004-1-AP targets ST8SIA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ST8SIA3 fusion protein Ag12497 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 3
Synonyms Alpha 2,8 sialyltransferase 8C, Sialyltransferase 8C, Sialytransferase St8Sia III, SIAT8 C, SIAT8C, ST8SIA3, ST8SiaIII
Calculated Molecular Weight 380 aa, 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC074909
Gene Symbol ST8SIA3
Gene ID (NCBI) 51046
RRIDAB_2239838
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43173
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The ST8 α-N-acetyl-neuraminide α-2,8-sialyltransferase (ST8Sia) family catalyzes the synthesis of one or multiple α2,8-linked sialic acid chains according to their acceptor specifificity (PMID:19888308). At least fifive ST8Sia enzymes (ST8SIA1, 2, 3, 4, and 6) have previously been identifified in adult brains (PMID:11530204). The ST8SIA3 is enriched in the striatum. The mouse St8sia3 gene shares 96% amino acid identity with human ST8SIA3, and is mainly expressed in the brain and testis (PMID:31455764).

实验方案

Product Specific Protocols
WB protocol for ST8SIA3 antibody 18004-1-APDownload protocol
IHC protocol for ST8SIA3 antibody 18004-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols