STAG1 Polyclonal antibody

STAG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14015-1-AP

Print datasheet

Synonyms

His SA, Stromal antigen 1, SCC3, SA1, Cohesin subunit SA-1



经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, human heart tissue, Raji cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

14015-1-AP targets STAG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Immunogen STAG1 fusion protein Ag5123 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name stromal antigen 1
Synonyms His SA, Stromal antigen 1, SCC3, SA1, Cohesin subunit SA-1
Calculated Molecular Weight 144 kDa
Observed Molecular Weight 155 kDa
GenBank Accession NumberBC064699
Gene Symbol STAG1
Gene ID (NCBI) 10274
RRIDAB_2194759
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WVM7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAG1, also named as SCC3 homolog 1, is a 1258 amino acid protein, which belongs to the SCC3 family. STAG1 interacts directly with RAD21 in cohesin complex and localizes in nucleus. STAG1 is a Component of cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The calculate molecular weight of STAG1 is 144 kDa, but modified STAG1 is about 145-150 kDa.

实验方案

Product Specific Protocols
WB protocol for STAG1 antibody 14015-1-APDownload protocol
IHC protocol for STAG1 antibody 14015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Bone Res

Circular RNA circStag1 promotes bone regeneration by interacting with HuR.

Authors - Gaoyang Chen
humanWB

Proc Natl Acad Sci U S A

Genetic analysis of cancer drivers reveals cohesin and CTCF as suppressors of PD-L1.

Authors - Ena Oreskovic