STAG1 Polyclonal antibody
STAG1 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, HeLa cells, human heart tissue, Raji cells, Jurkat cells, MCF-7 cells, NIH/3T3 cells |
Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
14015-1-AP targets STAG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human, rat |
Immunogen | STAG1 fusion protein Ag5123 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | stromal antigen 1 |
Synonyms | His SA, Stromal antigen 1, SCC3, SA1, Cohesin subunit SA-1 |
Calculated Molecular Weight | 144 kDa |
Observed Molecular Weight | 155 kDa |
GenBank Accession Number | BC064699 |
Gene Symbol | STAG1 |
Gene ID (NCBI) | 10274 |
RRID | AB_2194759 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q8WVM7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for STAG1 antibody 14015-1-AP | Download protocol |
IHC protocol for STAG1 antibody 14015-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Proc Natl Acad Sci U S A Genetic analysis of cancer drivers reveals cohesin and CTCF as suppressors of PD-L1. |