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STAG1 Polyclonal antibody, PBS Only

STAG1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 14015-1-PBS

Print datasheet

Synonyms

His SA, Stromal antigen 1, SCC3, SA1, Cohesin subunit SA-1

验证数据展示

  • WB analysis using 14015-1-AP (same clone as 14015-1-PBS)

    WB analysis using 14015-1-AP (same clone as 14015-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14015-1-AP (STAG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14015-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 14015-1-AP (same clone as 14015-1-PBS)

    IHC staining of human stomach using 14015-1-AP (same clone as 14015-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14015-1-AP (STAG1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14015-1-PBS in a different storage buffer formulation.

产品信息

14015-1-PBS targets STAG1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen STAG1 fusion protein Ag5123 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name stromal antigen 1
Synonyms His SA, Stromal antigen 1, SCC3, SA1, Cohesin subunit SA-1
Calculated Molecular Weight 144 kDa
Observed Molecular Weight 155 kDa
GenBank Accession NumberBC064699
Gene Symbol STAG1
Gene ID (NCBI) 10274
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WVM7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STAG1, also named as SCC3 homolog 1, is a 1258 amino acid protein, which belongs to the SCC3 family. STAG1 interacts directly with RAD21 in cohesin complex and localizes in nucleus. STAG1 is a Component of cohesin complex, which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. The calculate molecular weight of STAG1 is 144 kDa, but modified STAG1 is about 145-150 kDa.