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STAG3 Polyclonal antibody

STAG3 Polyclonal Antibody for IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 23314-1-AP

Print datasheet

Synonyms

Cohesin subunit SA 3, SCC3 homolog 3, STAG3, stromal antigen 3, Stromalin 3

验证数据展示

  • IP experiment of human testis using 23314-1-AP

    IP experiment of human testis using 23314-1-AP

    IP result of anti-STAG3 (IP:23314-1-AP, 4ug; Detection:23314-1-AP 1:300) with human testis tissue lysate 4000ug.

  • IHC staining of human testis using 23314-1-AP

    IHC staining of human testis using 23314-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23314-1-AP (STAG3 Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of A549 using 23314-1-AP

    IF Staining of A549 using 23314-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 23314-1-AP (STAG3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IP detected inhuman testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23314-1-AP is STAG3 Fusion Protein expressed in E. coli.

Tested Applications IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsIF, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen STAG3 fusion protein Ag19818 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name stromal antigen 3
Synonyms Cohesin subunit SA 3, SCC3 homolog 3, STAG3, stromal antigen 3, Stromalin 3
Calculated Molecular Weight 1225 aa, 139 kDa
Observed Molecular Weight 130-140 kDa
GenBank Accession NumberBC146806
Gene Symbol STAG3
Gene ID (NCBI) 10734
RRIDAB_2879253
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJ98
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for STAG3 antibody 23314-1-APDownload protocol
IF protocol for STAG3 antibody 23314-1-APDownload protocol
IP protocol for STAG3 antibody 23314-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Oncol

The Ectopic Expression of Meiosis Regulatory Genes in Cutaneous T-Cell Lymphomas (CTCL).

Authors - Jennifer Gantchev
View Article
humanIF

Front Genet

In-Frame Variants in STAG3 Gene Cause Premature Ovarian Insufficiency.

Authors - Wen-Juan Xiao
View Article