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STAM2 Polyclonal antibody

STAM2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 13009-1-AP

Print datasheet

Synonyms

STAM2A, STAM-2, STAM 2, Hrs-binding protein, Hrs binding protein

验证数据展示

  • WB analysis using 13009-1-AP

    WB analysis using 13009-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13009-1-AP (STAM2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 13009-1-AP

    IHC staining of mouse heart using 13009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13009-1-AP (STAM2 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with STAM2 antibody included
  • IHC staining of mouse kidney using 13009-1-AP

    IHC staining of mouse kidney using 13009-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13009-1-AP (STAM2 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with STAM2 antibody included
  • IF Staining of HeLa using 13009-1-AP

    IF Staining of HeLa using 13009-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using STAM2 antibody (13009-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 13009-1-AP

    WB analysis using 13009-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13009-1-AP (STAM2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, HepG2 cells
Positive IHC detected inmouse heart tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STAM2 Ready-To-Use IHC Kit. STAM2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13009-1-AP targets STAM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen STAM2 fusion protein Ag3698 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal transducing adaptor molecule (SH3 domain and ITAM motif) 2
Synonyms STAM2A, STAM-2, STAM 2, Hrs-binding protein, Hrs binding protein
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC028740
Gene Symbol STAM2
Gene ID (NCBI) 10254
RRIDAB_2197497
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75886
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for STAM2 antibody 13009-1-APDownload protocol
IHC protocol for STAM2 antibody 13009-1-APDownload protocol
IF protocol for STAM2 antibody 13009-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols