验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse brain tissue, HepG2 cells |
Positive IHC detected in | mouse heart tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13009-1-AP targets STAM2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | STAM2 fusion protein Ag3698 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 |
别名 | STAM2A, STAM-2, STAM 2, Hrs-binding protein, Hrs binding protein |
计算分子量 | 58 kDa |
观测分子量 | 68 kDa |
GenBank蛋白编号 | BC028740 |
基因名称 | STAM2 |
Gene ID (NCBI) | 10254 |
RRID | AB_2197497 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O75886 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for STAM2 antibody 13009-1-AP | Download protocol |
IHC protocol for STAM2 antibody 13009-1-AP | Download protocol |
IF protocol for STAM2 antibody 13009-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |