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STAR Recombinant antibody, PBS Only

STAR Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, pig, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5M6

Cat no : 80751-1-PBS

Print datasheet

Synonyms

START domain-containing protein 1, STARD1, 5M6

验证数据展示

  • WB analysis using 80751-1-RR (same clone as 80751-1-PBS)

    WB analysis using 80751-1-RR (same clone as 80751-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80751-1-RR (STAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • WB analysis using 80751-1-RR (same clone as 80751-1-PBS)

    WB analysis using 80751-1-RR (same clone as 80751-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80751-1-RR (STAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    IF Staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (80751-1-RR, Clone: 5M6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    IHC staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80751-1-RR (STAR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    IHC staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80751-1-RR (STAR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80751-1-PBS

    Affinity and Kinetic Characterization of 80751-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 80751-1-RR against Human STAR were performed. The affinity constant is 0.676 nM.

产品信息

80751-1-PBS targets STAR in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, rat, pig, mouse samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, rat, pig, mouse
Immunogen STAR fusion protein Ag2862 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name steroidogenic acute regulatory protein
Synonyms START domain-containing protein 1, STARD1, 5M6
Calculated Molecular Weight 285 aa, 32 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC010550
Gene Symbol STAR
Gene ID (NCBI) 6770
RRIDAB_2918910
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49675
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue.