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STAR Recombinant antibody, PBS Only

STAR Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, pig, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

5M6

Cat no : 80751-1-PBS

Print datasheet

Synonyms

START domain-containing protein 1, STARD1, 5M6

验证数据展示

  • WB analysis using 80751-1-RR (same clone as 80751-1-PBS)

    WB analysis using 80751-1-RR (same clone as 80751-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80751-1-RR (STAR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • WB analysis using 80751-1-RR (same clone as 80751-1-PBS)

    WB analysis using 80751-1-RR (same clone as 80751-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80751-1-RR (STAR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    IF Staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using STAR antibody (80751-1-RR, Clone: 5M6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    IHC staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80751-1-RR (STAR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    IHC staining of mouse testis using 80751-1-RR (same clone as 80751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 80751-1-RR (STAR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80751-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80751-1-PBS

    Affinity and Kinetic Characterization of 80751-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 80751-1-RR against Human STAR were performed. The affinity constant is 0.676 nM.

产品信息

80751-1-PBS targets STAR in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, rat, pig, mouse samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, rat, pig, mouse
Immunogen STAR fusion protein Ag2862 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name steroidogenic acute regulatory protein
Synonyms START domain-containing protein 1, STARD1, 5M6
Calculated Molecular Weight 285 aa, 32 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC010550
Gene Symbol STAR
Gene ID (NCBI) 6770
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49675
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Steroidogenic acute regulatory protein (StAR) is a key player in acute regulation of hormone-induced steroidogenesis, by facilitating cholesterol transport from cellular stores to inner mitochondria membrane where the cholesterol was conversed to pregnenolone. StAR is prevalently expressed in mitochondria of steroid-producing adrenal and gonadal tissue.