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验证数据展示

  • WB analysis using 68554-1-Ig

    WB analysis using 68554-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68554-1-Ig (STARD10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 68554-1-Ig

    IP experiment of HepG2 using 68554-1-Ig

    IP result of anti-STARD10 (IP:68554-1-Ig, 4ug; Detection:68554-1-Ig 1:6000) with HepG2 cells lysate 1480 ug.

  • IF Staining of HeLa using 68554-1-Ig

    IF Staining of HeLa using 68554-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using STARD10 antibody (68554-1-Ig, Clone: 1B9A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

STARD10 Monoclonal antibody

STARD10 Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 68554-1-Ig

产品说明书

CloneNo. 1B9A1

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, rabbit, pig

应用

WB, IF/ICC, IP, ELISA

别名

Antigen NY CO 28, CGI 52, NY CO 28, PCTP L, PCTP like protein, PCTP2, SDCCAG28, STARD10

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSK-BR-3 cells, MCF-7 cells, T-47D cells, LNCaP cells, rat liver tissue, mouse liver tissue, pig liver tissue, rabbit liver tissue, rabbit testis tissue
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68554-1-Ig targets STARD10 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 Human, mouse, rat, rabbit, pig
免疫原 STARD10 fusion protein Ag10932 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 StAR-related lipid transfer (START) domain containing 10
别名 Antigen NY CO 28, CGI 52, NY CO 28, PCTP L, PCTP like protein, PCTP2, SDCCAG28, STARD10
计算分子量 291 aa, 33 kDa
观测分子量35-40 kDa
GenBank蛋白编号BC007919
基因名称 STARD10
Gene ID (NCBI) 10809
RRIDAB_3085257
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9Y365
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STARD10 (START domain-containing protein 10, also known as PCTP-like protein) is highly expressed in liver and responsible to transfer phosphatidylcholine. STARD10 was identified as a protein overexpressed in breast cancer that cooperates with the ErbB family of receptor tyrosine kinases in cellular transformation (PMID: 15911624). This antibody detected duplicate bands between 35-40 kDa (PMID: 15150109).

实验方案

Product Specific Protocols
WB protocol for STARD10 antibody 68554-1-IgDownload protocol
IF protocol for STARD10 antibody 68554-1-IgDownload protocol
IP protocol for STARD10 antibody 68554-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols