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STARD10 Monoclonal antibody

STARD10 Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1B9A1

Cat no : 68554-1-Ig

Print datasheet

Synonyms

Antigen NY CO 28, CGI 52, NY CO 28, PCTP L, PCTP like protein, PCTP2, SDCCAG28, STARD10

验证数据展示

  • WB analysis using 68554-1-Ig

    WB analysis using 68554-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68554-1-Ig (STARD10 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 68554-1-Ig

    IP experiment of HepG2 using 68554-1-Ig

    IP result of anti-STARD10 (IP:68554-1-Ig, 4ug; Detection:68554-1-Ig 1:6000) with HepG2 cells lysate 1480 ug.

  • IF Staining of HeLa using 68554-1-Ig

    IF Staining of HeLa using 68554-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using STARD10 antibody (68554-1-Ig, Clone: 1B9A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inSK-BR-3 cells, MCF-7 cells, T-47D cells, LNCaP cells, rat liver tissue, mouse liver tissue, pig liver tissue, rabbit liver tissue, rabbit testis tissue
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68554-1-Ig targets STARD10 in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity Human, mouse, rat, rabbit, pig
Immunogen STARD10 fusion protein Ag10932 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name StAR-related lipid transfer (START) domain containing 10
Synonyms Antigen NY CO 28, CGI 52, NY CO 28, PCTP L, PCTP like protein, PCTP2, SDCCAG28, STARD10
Calculated Molecular Weight 291 aa, 33 kDa
Observed Molecular Weight35-40 kDa
GenBank Accession NumberBC007919
Gene Symbol STARD10
Gene ID (NCBI) 10809
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y365
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STARD10 (START domain-containing protein 10, also known as PCTP-like protein) is highly expressed in liver and responsible to transfer phosphatidylcholine. STARD10 was identified as a protein overexpressed in breast cancer that cooperates with the ErbB family of receptor tyrosine kinases in cellular transformation (PMID: 15911624). This antibody detected duplicate bands between 35-40 kDa (PMID: 15150109).

实验方案

Product Specific Protocols
WB protocol for STARD10 antibody 68554-1-IgDownload protocol
IF protocol for STARD10 antibody 68554-1-IgDownload protocol
IP protocol for STARD10 antibody 68554-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols