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STAT4 Monoclonal antibody, PBS Only

STAT4 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4A8C9

Cat no : 67568-2-PBS

Print datasheet

Synonyms

SLEB11, DPMC, 4A8C9

验证数据展示

  • WB analysis using 67568-2-Ig (same clone as 67568-2-PBS)

    WB analysis using 67568-2-Ig (same clone as 67568-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67568-2-Ig (STAT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 67568-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67568-2-Ig (same clone as 67568-2-PBS)

    IF Staining of HepG2 using 67568-2-Ig (same clone as 67568-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67568-2-Ig (STAT4 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67568-2-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67568-2-Ig (same clone as 67568-2-PBS)

    IHC staining of human breast cancer using 67568-2-Ig (same clone as 67568-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67568-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STAT4 antibody included
  • IHC staining of human breast cancer using 67568-2-Ig (same clone as 67568-2-PBS)

    IHC staining of human breast cancer using 67568-2-Ig (same clone as 67568-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67568-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STAT4 antibody included
  • FC experiment of HepG2 using 67568-2-Ig (same clone as 67568-2-PBS)

    FC experiment of HepG2 using 67568-2-Ig (same clone as 67568-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug STAT4 Monoclonal antibody (67568-2-Ig, Clone:4A8C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67568-2-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy STAT4 Ready-To-Use IHC Kit. STAT4 primary antibody included.

产品信息

67568-2-PBS targets STAT4 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen STAT4 fusion protein Ag19545 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name signal transducer and activator of transcription 4
Synonyms SLEB11, DPMC, 4A8C9
Calculated Molecular Weight 748 aa, 86 kDa
Observed Molecular Weight 86 kDa
GenBank Accession NumberBC031212
Gene Symbol STAT4
Gene ID (NCBI) 6775
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14765
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209].