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STAT5B Monoclonal antibody, PBS Only

STAT5B Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D9B11

Cat no : 66427-1-PBS

Print datasheet

Synonyms

STAT5A/B, STAT5, 1D9B11

验证数据展示

  • WB analysis of Jurkat using 66427-1-Ig (same clone as 66427-1-PBS)

    WB analysis of Jurkat using 66427-1-Ig (same clone as 66427-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66427-1-Ig (STAT5B Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66427-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66427-1-Ig (same clone as 66427-1-PBS)

    IF Staining of HepG2 using 66427-1-Ig (same clone as 66427-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using STAT5B antibody (66427-1-Ig, Clone: 1D9B11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66427-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66427-1-Ig (same clone as 66427-1-PBS)

    FC experiment of HeLa using 66427-1-Ig (same clone as 66427-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.5 ug Anti-Human STAT5B (66427-1-Ig, Clone:1D9B11) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG2a (KFA042), or 0.5 ug Mouse IgG2a Isotype Control (66360-2-Ig, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66427-1-PBS in a different storage buffer formulation.

产品信息

66427-1-PBS targets STAT5B in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen STAT5B fusion protein Ag2709 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name signal transducer and activator of transcription 5B
Synonyms STAT5A/B, STAT5, 1D9B11
Calculated Molecular Weight 787 aa, 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC020868
Gene Symbol STAT5B
Gene ID (NCBI) 6777
ENSEMBL Gene IDENSG00000173757
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51692
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STAT5B belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5B carries out a dual function: signal transduction and activation of transcription. STAT5B mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different GH. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. Despite the 95% homology between the immunogen (Ag2709) and the STAT5A protein, our WB detection showed that this antibody does not recognize the STAT5A fusion protein (Ag19546).