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STBD1 Monoclonal antibody

STBD1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

CloneNo.

1A2G2

Cat no : 67018-1-Ig

Print datasheet

Synonyms

Starch-binding domain-containing protein 1, starch binding domain 1, Glycophagy cargo receptor STBD1, GENX-3414, Genethonin-1

验证数据展示

  • WB analysis using 67018-1-Ig

    WB analysis using 67018-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67018-1-Ig (STBD1 antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • IHC staining of human liver cancer using 67018-1-Ig

    IHC staining of human liver cancer using 67018-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67018-1-Ig (STBD1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STBD1 antibody included
  • IHC staining of human liver cancer using 67018-1-Ig

    IHC staining of human liver cancer using 67018-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67018-1-Ig (STBD1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STBD1 antibody included
  • IF Staining of HeLa using 67018-1-Ig

    IF Staining of HeLa using 67018-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using STBD1 antibody (67018-1-Ig, Clone: 1A2G2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, A549 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy STBD1 Ready-To-Use IHC Kit. STBD1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67018-1-Ig targets STBD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen STBD1 fusion protein Ag28613 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name starch binding domain 1
Synonyms Starch-binding domain-containing protein 1, starch binding domain 1, Glycophagy cargo receptor STBD1, GENX-3414, Genethonin-1
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC022301
Gene Symbol STBD1
Gene ID (NCBI) 8987
RRIDAB_2882334
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO95210
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STBD1 may have the capability to bind to carbohydrates. It functions as a glycogen receptor that tethers glycogen to autophagic membranes for delivery and breakdown in lysosomes. STBD1 appears molecular mass of 35-38 kDa band in mouse/rat tissues and 38-43 kDa in human tissue.

实验方案

Product Specific Protocols
WB protocol for STBD1 antibody 67018-1-IgDownload protocol
IHC protocol for STBD1 antibody 67018-1-IgDownload protocol
IF protocol for STBD1 antibody 67018-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF,IP

J Clin Invest

Aryl hydrocarbon receptor sulfenylation promotes glycogenolysis and rescues cancer chemoresistance

Authors - Nannan Zhou
View Article
humanIHC

Nat Commun

Model-based analysis uncovers mutations altering autophagy selectivity in human cancer.

Authors - Zhu Han
View Article