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STEAP1 Polyclonal antibody

STEAP1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20199-1-AP

Print datasheet

Synonyms

Metalloreductase STEAP1, PRSS24, STEAP, STEAP1

验证数据展示

  • WB analysis of A431 using 20199-1-AP

    WB analysis of A431 using 20199-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 20199-1-AP (STEAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 20199-1-AP

    IHC staining of human prostate cancer using 20199-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20199-1-AP (STEAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 20199-1-AP

    IHC staining of human prostate cancer using 20199-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 20199-1-AP (STEAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA431 cells, DU 145 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20199-1-AP targets STEAP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen STEAP1 fusion protein Ag14134 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name six transmembrane epithelial antigen of the prostate 1
Synonyms Metalloreductase STEAP1, PRSS24, STEAP, STEAP1
Calculated Molecular Weight 339 aa, 40 kDa
Observed Molecular Weight 38-40 kDa
GenBank Accession NumberBC011802
Gene Symbol STEAP1
Gene ID (NCBI) 26872
RRIDAB_11182488
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHE8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for STEAP1 antibody 20199-1-APDownload protocol
IHC protocol for STEAP1 antibody 20199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Death Dis

METTL3-mediated m6A modification of STEAP2 mRNA inhibits papillary thyroid cancer progress by blocking the Hedgehog signaling pathway and epithelial-to-mesenchymal transition.

Authors - Yue Zhu
View Article
humanWB

Cancer Biol Ther

STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2

Authors - Dong Zhang
View Article