• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IHC staining of human colon cancer using 24804-1-AP

    IHC staining of human colon cancer using 24804-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24804-1-AP (STEAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 24804-1-AP

    IHC staining of human colon cancer using 24804-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24804-1-AP (STEAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 24804-1-AP

    WB analysis using 24804-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24804-1-AP (STEAP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

STEAP2 Polyclonal antibody

STEAP2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24804-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

PUMPCn, Protein up-regulated in metastatic prostate cancer, Prostate cancer-associated protein 1, PCANAP1, Metalloreductase STEAP2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inDU 145 cells, HEK-293T cells, U2OS cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:800-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24804-1-AP targets STEAP2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen STEAP2 fusion protein Ag20293 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name six transmembrane epithelial antigen of the prostate 2
Synonyms PUMPCn, Protein up-regulated in metastatic prostate cancer, Prostate cancer-associated protein 1, PCANAP1, Metalloreductase STEAP2
Calculated Molecular Weight 490 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC148824
Gene Symbol STEAP2
Gene ID (NCBI) 261729
RRIDAB_3085755
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFT2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STEAP2, also named as PCANAP1 and STAMP1, belongs to the STEAP family. It is a metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. STEAP2 uses NAD+ as acceptor.

实验方案

Product Specific Protocols
WB protocol for STEAP2 antibody 24804-1-APDownload protocol
IHC protocol for STEAP2 antibody 24804-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols