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  • Featured Product
  • KD/KO Validated

STEAP2 Polyclonal antibody

STEAP2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20201-1-AP

Print datasheet

Synonyms

STEAP2-Specific, PUMPCn, Protein up-regulated in metastatic prostate cancer, Prostate cancer-associated protein 1, PCANAP1

验证数据展示

  • WB analysis of HEK-293 using 20201-1-AP

    WB analysis of HEK-293 using 20201-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20201-1-AP (STEAP2-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 20201-1-AP

    WB analysis of HeLa using 20201-1-AP

    WB result of STEAP2-Specific antibody (20201-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-STEAP2-Specific transfected HeLa cells.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, DU 145 cells, fetal human brain tissue, mouse kidney tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20201-1-AP targets STEAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name six transmembrane epithelial antigen of the prostate 2
Synonyms STEAP2-Specific, PUMPCn, Protein up-regulated in metastatic prostate cancer, Prostate cancer-associated protein 1, PCANAP1
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 48-52 kDa
GenBank Accession NumberNM_152999
Gene Symbol STEAP2
Gene ID (NCBI) 261729
RRIDAB_10665556
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NFT2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STEAP2, also named as PCANAP1 and STAMP1, belongs to the STEAP family. It is a metalloreductase that has the ability to reduce both Fe3+ to Fe2+ and Cu2+ to Cu1+. STEAP2 uses NAD+ as acceptor. The antibody is specific to STEAP2.

实验方案

Product Specific Protocols
WB protocol for STEAP2 antibody 20201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Exp Cell Res

Membrane cholesterol modulates STEAP2 conformation during dynamic intracellular trafficking processes leading to broad subcellular distribution.

Authors - Haruki Hasegawa
View Article
humanWB,IHC

Cell Death Dis

METTL3-mediated m6A modification of STEAP2 mRNA inhibits papillary thyroid cancer progress by blocking the Hedgehog signaling pathway and epithelial-to-mesenchymal transition.

Authors - Yue Zhu
  • KD Validated
View Article
humanWB

Cancer Biol Ther

STEAP2 promotes osteosarcoma progression by inducing epithelial–mesenchymal transition via the PI3K/AKT/mTOR signaling pathway and is regulated by EFEMP2

Authors - Dong Zhang
  • KD Validated
View Article
mouseWB

J Cell Mol Med

Unveiling the role of copper metabolism and STEAP2 in idiopathic pulmonary fibrosis molecular landscape

Authors - Yajun Wang
  • KD Validated
View Article