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STEAP3 Polyclonal antibody

STEAP3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 28478-1-AP

Print datasheet

Synonyms

dudlin 2, Dudulin 2, hpHyde, hTSAP6, Metalloreductase STEAP3, pHyde, STEAP family member 3, STEAP3, STMP3, TSAP6

验证数据展示

  • WB analysis using 28478-1-AP

    WB analysis using 28478-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28478-1-AP (STEAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human appendicitis using 28478-1-AP

    IHC staining of human appendicitis using 28478-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STEAP3 antibody included
  • IHC staining of human appendicitis using 28478-1-AP

    IHC staining of human appendicitis using 28478-1-AP

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STEAP3 antibody included
  • IHC staining of human urothelial carcinoma using 28478-1-AP

    IHC staining of human urothelial carcinoma using 28478-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STEAP3 antibody included
  • IHC staining of human urothelial carcinoma using 28478-1-AP

    IHC staining of human urothelial carcinoma using 28478-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STEAP3 antibody included
  • IHC staining of mouse liver using 28478-1-AP

    IHC staining of mouse liver using 28478-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28478-1-AP (STEAP3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STEAP3 antibody included
  • IF Staining of HepG2 using 28478-1-AP

    IF Staining of HepG2 using 28478-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using STEAP3 antibody (28478-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, PC-12 cells, C6 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman appendicitis tissue, mouse liver tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy STEAP3 Ready-To-Use IHC Kit. STEAP3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28478-1-AP targets STEAP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen STEAP3 fusion protein Ag29528 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name STEAP family member 3
Synonyms dudlin 2, Dudulin 2, hpHyde, hTSAP6, Metalloreductase STEAP3, pHyde, STEAP family member 3, STEAP3, STMP3, TSAP6
Calculated Molecular Weight 488 aa, 55 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC042150
Gene Symbol STEAP3
Gene ID (NCBI) 55240
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ658P3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STEAP3 (Six-Transmembrane Epithelial Antigen of Prostate 3) is also named as TSAP6, Dudulin-2 and pHyde, and belongs to the STEAP family. STEAP3 is a member of the STEAP family and is composed of a six-transmembrane domain at the COOH-terminal domain and a cytoplasmic N-terminal oxidoreductase domain, which is essential for iron and copper uptake (PMID:16227996). STEAP3 contains a functional p53-binding site in its promoter and can be upregulated following p53 activation to enhance cell death in myeloid leukemia cell line and breast cancer cells (PMID: 18617898). By interacting with Nix, a pro-apoptotic Bcl-2 family member, and Myt1 kinase, a negative regulator of the G2/M transition, STEAP3 overexpression promotes apoptosis and inhibits G2/M transition in cell cycle progression (PMID: 12606722, PMID: 10504341).

实验方案

Product Specific Protocols
WB protocol for STEAP3 antibody 28478-1-APDownload protocol
IHC protocol for STEAP3 antibody 28478-1-APDownload protocol
IF protocol for STEAP3 antibody 28478-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols