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STEAP4 Polyclonal antibody

STEAP4 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 11944-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, ELISA and More (1)

TNFAIP9, STEAP family member 4, STAMP2, Six-transmembrane epithelial antigen of prostate 4, Metalloreductase STEAP4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, 3T3-L1 cells, rat liver tissue, human liver tissue, human brain tissue, RAW 264.7 cells
Positive IP detected in3T3-L1 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11944-1-AP targets STEAP4 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 STEAP4 fusion protein Ag2545 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 STEAP family member 4
别名 TNFAIP9, STEAP family member 4, STAMP2, Six-transmembrane epithelial antigen of prostate 4, Metalloreductase STEAP4
计算分子量 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC020600
基因名称 STEAP4
Gene ID (NCBI) 79689
RRIDAB_2197868
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ687X5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STEAP4 (also called STAMP2), is a member of the STEAP family. Structurally, It is a 459 amino acid protein characterized by a molecular topology of six transmembrane domains. The cytoplasmic N-terminal domain of STEAP4 shows structural similarity with bacterial and archaeal FNO oxidoreductase and STEAP4 exhibits a strong iron reductase activity. Studies in mice and human suggest that STEAP4 maybe involved in adipocyte development and metabolism, and may contribute to the normal biology of the prostate cell, as well as prostate cancer progression.

实验方案

Product Specific Protocols
WB protocol for STEAP4 antibody 11944-1-APDownload protocol
IHC protocol for STEAP4 antibody 11944-1-APDownload protocol
IF protocol for STEAP4 antibody 11944-1-APDownload protocol
IP protocol for STEAP4 antibody 11944-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

J Allergy Clin Immunol

Six-transmembrane epithelial antigens of the prostate comprise a novel inflammatory nexus in patients with pustular skin disorders.

Authors - Yun Liang
mouse,ratWB,IHC

Bone Res

Dietary fat-associated osteoarthritic chondrocytes gain resistance to lipotoxicity through PKCK2/STAMP2/FSP27.

Authors - Sung Won Lee
  • KD Validated
mouse,human,ratIHC,WB

Basic Res Cardiol

The six-transmembrane protein Stamp2 ameliorates pulmonary vascular remodeling and pulmonary hypertension in mice.

Authors - Mehreen Batool
  • KO Validated
humanWB,IHC

Nat Commun

Inflammation mobilizes copper metabolism to promote colon tumorigenesis via an IL-17-STEAP4-XIAP axis.

Authors - Yun Liao
mouseIF

Proc Natl Acad Sci U S A

Quantitative proteomics identifies STEAP4 as a critical regulator of mitochondrial dysfunction linking inflammation and colon cancer.

Authors - Xiang Xue
human, mouseWB, IHC

J Hepatol

PMID: 25646886

Authors - Hye Y Kim
  • KD Validated
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