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  • Featured Product
  • KD/KO Validated

STK10 Polyclonal antibody

STK10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25471-1-AP

Print datasheet

Synonyms

serine/threonine kinase 10, PRO2729, Lymphocyte-oriented kinase, Lymphocyte oriented kinase, LOK

验证数据展示

  • IHC staining of human lymphoma using 25471-1-AP

    IHC staining of human lymphoma using 25471-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lymphoma using 25471-1-AP

    IHC staining of human lymphoma using 25471-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 25471-1-AP (STK10 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of SKOV-3 using 25471-1-AP

    WB analysis of SKOV-3 using 25471-1-AP

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 25471-1-AP (STK10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inSKOV-3 cells, A549 cells, COLO 320 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25471-1-AP targets STK10 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen STK10 fusion protein Ag22169 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serine/threonine kinase 10
Synonyms serine/threonine kinase 10, PRO2729, Lymphocyte-oriented kinase, Lymphocyte oriented kinase, LOK
Calculated Molecular Weight 968 aa, 112 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC070077
Gene Symbol STK10
Gene ID (NCBI) 6793
RRIDAB_2880096
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94804
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lymphocyte-oriented kinase deficiency encoded by the serine/threonine kinase 10 (STK10) gene correlates with the intracellular adhesion molecule 1 (ICAM-1)/lymphocyte function associated antigen 1 (LFA-1) complex in aspirin hypersensitivity(PMID:21905501). STK 10 is also named as LOK and expressed as a 130-kDa protein, which was detected predominantly in lymphoid organs such as spleen, thymus, and bone marrow, in contrast to other mammalian members of the STE20 family(PMID:9278426).

实验方案

Product Specific Protocols
WB protocol for STK10 antibody 25471-1-APDownload protocol
IHC protocol for STK10 antibody 25471-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Immunol

The enhancement of immunoactivity induced by immunogenic cell death through serine/threonine kinase 10 inhibition: a potential therapeutic strategy

Authors - Xiaoli Xia
  • KD Validated
View Article

Clin Cancer Res

Combined CDK4/6 and ERK1/2 inhibition enhances anti-tumor activity in NF1-associated plexiform neurofibroma

Authors - Alyssa C Flint
View Article