验证数据展示
经过测试的应用
Positive WB detected in | HepG2 cells |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human lung cancer tissue, human testis tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10746-1-AP targets STK11/LKB1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP, CoIP |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse, rat, pig, hamster |
免疫原 | STK11/LKB1 fusion protein Ag1048 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | serine/threonine kinase 11 |
别名 | LKB1, STK11, STK11/ LKB1, Renal carcinoma antigen NY-REN-19, PJS |
计算分子量 | 49 kDa |
观测分子量 | 50-55 kDa |
GenBank蛋白编号 | BC007981 |
基因名称 | LKB1 |
Gene ID (NCBI) | 6794 |
RRID | AB_2271311 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q15831 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers. STK11 has 2 isoforms with MW of 49 kDa and 45 kDa, and can be detected as 50-54 kDa after posttranslational modification.
实验方案
Product Specific Protocols | |
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WB protocol for STK11/LKB1 antibody 10746-1-AP | Download protocol |
IHC protocol for STK11/LKB1 antibody 10746-1-AP | Download protocol |
IP protocol for STK11/LKB1 antibody 10746-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Metab CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.
| ||
Nat Commun Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents. | ||
EBioMedicine Exogenous NADPH exerts a positive inotropic effect and enhances energy metabolism via SIRT3 in pathological cardiac hypertrophy and heart failure | ||
Kidney Int Rho-associated, coiled-coil-containing protein kinase 1 regulates development of diabetic kidney disease via modulation of fatty acid metabolism. | ||
Aging Cell Antagonizing peroxisome proliferator-activated receptor γ facilitates M1-to-M2 shift of microglia by enhancing autophagy via the LKB1-AMPK signaling pathway. |