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STK11/LKB1 Monoclonal antibody

STK11/LKB1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D11E5

Cat No : 68016-1-Ig

Print datasheet

Synonyms

LKB1, PJS, serine/threonine kinase 11, STK11

验证数据展示

  • WB analysis of HEK-293 using 68016-1-Ig

    WB analysis of HEK-293 using 68016-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:4900 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 68016-1-Ig

    IHC staining of mouse testis using 68016-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STK11 antibody included
  • WB analysis of NIH/3T3 using 68016-1-Ig

    WB analysis of NIH/3T3 using 68016-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68016-1-Ig (STK11/LKB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse colon using 68016-1-Ig

    IF Staining of mouse colon using 68016-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using STK11/LKB1 antibody (68016-1-Ig, Clone: 2D11E5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, Calyculin A treated HSC-T6 cells, NIH/3T3 cells, PC-3 cells, HeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy STK11 Ready-To-Use IHC Kit. STK11 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:9800
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68016-1-Ig targets STK11/LKB1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name serine/threonine kinase 11
Synonyms LKB1, PJS, serine/threonine kinase 11, STK11
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC007981
Gene Symbol STK11
Gene ID (NCBI) 6794
RRIDAB_2918762
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ15831
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers. STK11 has 2 isoforms with MW of 49 kDa and 45 kDa, and can be detected as 50-54 kDa after posttranslational modification.

实验方案

Product Specific Protocols
WB protocol for STK11/LKB1 antibody 68016-1-IgDownload protocol
IHC protocol for STK11/LKB1 antibody 68016-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols