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STK32B Polyclonal antibody

STK32B Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15438-1-AP

Print datasheet

Synonyms

STKG6, STK32, Serine/threonine-protein kinase 32B, serine/threonine kinase 32B, HSA250839

验证数据展示

  • WB analysis using 15438-1-AP

    WB analysis using 15438-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15438-1-AP (STK32B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of U2OS using 15438-1-AP

    IF Staining of U2OS using 15438-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using STK32B antibody (15438-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, SH-SY5Y cells
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

15438-1-AP targets STK32B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsIHC
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen STK32B fusion protein Ag4507 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serine/threonine kinase 32B
Synonyms STKG6, STK32, Serine/threonine-protein kinase 32B, serine/threonine kinase 32B, HSA250839
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 43-48 kDa
GenBank Accession NumberBC038238
Gene Symbol STK32B
Gene ID (NCBI) 55351
RRIDAB_2196612
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NY57
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for STK32B antibody 15438-1-APDownload protocol
IF protocol for STK32B antibody 15438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Cell Rep

Two parabrachial Cck neurons involved in the feedback control of thirst or salt appetite

Authors - Takashi Matsuda
View Article