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STK33 Polyclonal antibody

STK33 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12857-1-AP

Print datasheet

Synonyms

Serine/threonine-protein kinase 33, serine/threonine kinase 33, EC:2.7.11.1

验证数据展示

  • WB analysis of human kidney using 12857-1-AP

    WB analysis of human kidney using 12857-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12857-1-AP (STK33 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 12857-1-AP

    IP experiment of HEK-293 using 12857-1-AP

    IP result of anti-STK33 (IP:12857-1-AP, 4ug; Detection:12857-1-AP 1:300) with HEK-293 cells lysate 880ug.

  • IHC staining of human liver cancer using 12857-1-AP

    IHC staining of human liver cancer using 12857-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12857-1-AP (STK33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A431 using 12857-1-AP

    IF Staining of A431 using 12857-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using STK33 antibody (12857-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inhuman kidney tissue, mouse testis tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12857-1-AP targets STK33 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen STK33 fusion protein Ag3561 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serine/threonine kinase 33
Synonyms Serine/threonine-protein kinase 33, serine/threonine kinase 33, EC:2.7.11.1
Calculated Molecular Weight 514 aa, 58 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC031231
Gene Symbol STK33
Gene ID (NCBI) 65975
RRIDAB_10642563
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYT3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serine/threonine kinase 33 (STK33) gene, which is identified in human chromosome 11 and mouse chromosome 7, encodes a serine/threonine kinase. STK33 belongs to a member of the calcium/calmodulin‐dependent kinase (CAMK), which is mainly existed in testis, certain brain regions and embryonic organs such as brain, heart and spinal cord in human and mouse and is likely to participate in spermatogenesis and organ ontogenesis. (PMID: 30256516, PMID: 34536775)

实验方案

Product Specific Protocols
WB protocol for STK33 antibody 12857-1-APDownload protocol
IHC protocol for STK33 antibody 12857-1-APDownload protocol
IF protocol for STK33 antibody 12857-1-APDownload protocol
IP protocol for STK33 antibody 12857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cell Proteomics

STK33 phosphorylates fibrous sheath protein AKAP3/4 to regulate sperm flagella assembly in spermiogenesis

Authors - Weiling Yu
View Article