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  • Featured Product
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STK38 Polyclonal antibody

STK38 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11105-1-AP

Print datasheet

Synonyms

Nuclear Dbf2-related kinase 1, Nuclear Dbf2 related kinase 1, NDR1 protein kinase, NDR1, NDR

验证数据展示

  • WB analysis of HeLa using 11105-1-AP

    WB analysis of HeLa using 11105-1-AP

    WB result of STK38 antibody (11105-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-STK38 transfected HeLa cells.

  • IP experiment of MCF-7 using 11105-1-AP

    IP experiment of MCF-7 using 11105-1-AP

    IP result of anti-STK38 (IP:11105-1-AP, 4ug; Detection:11105-1-AP 1:300) with MCF-7 cells lysate 800ug.

  • WB analysis using 11105-1-AP

    WB analysis using 11105-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11105-1-AP (STK38 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 11105-1-AP

    IHC staining of mouse cerebellum using 11105-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11105-1-AP (STK38 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STK38 antibody included
  • IHC staining of mouse cerebellum using 11105-1-AP

    IHC staining of mouse cerebellum using 11105-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11105-1-AP (STK38 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STK38 antibody included

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy STK38 Ready-To-Use IHC Kit. STK38 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11105-1-AP targets STK38 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen STK38 fusion protein Ag1559 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name serine/threonine kinase 38
Synonyms Nuclear Dbf2-related kinase 1, Nuclear Dbf2 related kinase 1, NDR1 protein kinase, NDR1, NDR
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC012085
Gene Symbol STK38
Gene ID (NCBI) 11329
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15208
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serine/threonine-protein kinase 38 belongs to AGC Ser/Thr protein kinase family.STK38 implicates in cell proliferation and/or tumor progression, and might play a role in the HIV-1 life cycle.Researches showed that STK38 with STK38L, were proapoptotic kinases and key members of the RASSF1/STK4 signaling cascade and its kinase activity increased in early mitotic phase and was dependent on FRY and STK3.This antibody recognizes the C-terminal of STK38.

实验方案

Product Specific Protocols
WB protocol for STK38 antibody 11105-1-APDownload protocol
IHC protocol for STK38 antibody 11105-1-APDownload protocol
IP protocol for STK38 antibody 11105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Nat Commun

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Authors - Xin Li
View Article