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验证数据展示

  • WB analysis of MCF-7 using 55335-1-AP

    WB analysis of MCF-7 using 55335-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 55335-1-AP (STK38 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 55335-1-AP

    IHC staining of human kidney using 55335-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with STK38 antibody included
  • IHC staining of human kidney using 55335-1-AP

    IHC staining of human kidney using 55335-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55335-1-AP (STK38 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with STK38 antibody included
  • IF Staining of HEK-293 using 55335-1-AP

    IF Staining of HEK-293 using 55335-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STK38 antibody (55335-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

STK38 Polyclonal antibody

STK38 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 55335-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (2)

别名

Nuclear Dbf2-related kinase 1, Nuclear Dbf2 related kinase 1, NDR1 protein kinase, NDR1, NDR

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells
Positive IHC detected inhuman kidney tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy STK38 Ready-To-Use IHC Kit. STK38 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55335-1-AP targets STK38 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serine/threonine kinase 38
别名 Nuclear Dbf2-related kinase 1, Nuclear Dbf2 related kinase 1, NDR1 protein kinase, NDR1, NDR
计算分子量 54 kDa
观测分子量 54 kDa
GenBank蛋白编号NM_007271
基因名称 STK38
Gene ID (NCBI) 11329
RRIDAB_2881310
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15208
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Serine/threonine-protein kinase 38 belongs to AGC Ser/Thr protein kinase family.STK38 implicates in cell proliferation and/or tumor progression, and might play a role in the HIV-1 life cycle.Researches showed that STK38 with STK38L, were proapoptotic kinases and key members of the RASSF1/STK4 signaling cascade and its kinase activity increased in early mitotic phase and was dependent on FRY and STK3.This antibody recognizes the C-terminal of STK38.

实验方案

Product Specific Protocols
WB protocol for STK38 antibody 55335-1-APDownload protocol
IHC protocol for STK38 antibody 55335-1-APDownload protocol
IF protocol for STK38 antibody 55335-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF, WB, IHC

J Cell Biochem

Phosphorylated LASS2 inhibits prostate carcinogenesis via negative regulation of Wnt/β-catenin signaling.

Authors - Kuangen Zhang
View Article
humanWB,IF

J Proteome Res

ZSWIM1 Promotes the Proliferation and Metastasis of Lung Adenocarcinoma Cells through the STK38/MEKK2/ERK1/2 Axis

Authors - Xuejuan Gao
View Article
humanWB,IP

Nat Commun

A bacterial autotransporter impairs innate immune responses by targeting the transcription factor TFE3

Authors - Atri Ta
View Article